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Literature summary for 2.7.2.8 extracted from

  • Haas, D.; Leisinger, T.
    N-acetylglutamate 5-phosphotransferase of Pseudomonas aeruginosa. Catalytic and regulatory properties (1975), Eur. J. Biochem., 52, 377-383.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
Pseudomonas aeruginosa
arginine feedback inhibition is markedly dependent on pH, above pH 9 no inhibition Pseudomonas aeruginosa
L-citrulline
-
Pseudomonas aeruginosa
MgCl2 30 mM, slight inhibition Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2
-
N-acetyl-L-glutamate pH 7.2, 37°C Pseudomonas aeruginosa
3
-
ATP pH 7.2, 37°C Pseudomonas aeruginosa

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ Pseudomonas aeruginosa
Co2+ Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ Pseudomonas aeruginosa
Mg2+ required Pseudomonas aeruginosa
Mg2+ 30 mM, slight inhibition Pseudomonas aeruginosa
Mn2+ Mn2+, Zn2+, Co2+ and Ca2+ in decreasing order can partially replace Mg2+ Pseudomonas aeruginosa
Zn2+ Mn2+, Zn2+, Co2+ and Ca2+ in this order can partially replace Mg2+ Pseudomonas aeruginosa

Organism

Organism UniProt Comment Textmining
Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + N-acetyl-L-glutamate
-
Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate in presence of hydroxylamine formation of N-acetyl-L-glutamate 5-hydroxamate + ADP + phosphate ?
ATP + N-carbamoyl-L-glutamate at 33% of the activity with N-acetyl-L-glutamate Pseudomonas aeruginosa ADP + N-carbamoyl-L-glutamate 5-phosphate
-
?
ATP + N-formyl-L-glutamate at 20% of the activity with N-acetyl-L-glutamate Pseudomonas aeruginosa ADP + N-formyl-L-glutamate 5-phosphate
-
?
dATP + N-acetyl-L-glutamate as effective as ATP Pseudomonas aeruginosa dADP + N-acetyl-L-glutamate 5-phosphate
-
?
additional information not: ITP Pseudomonas aeruginosa ?
-
?
additional information not: GTP Pseudomonas aeruginosa ?
-
?
additional information N-propionyl-L-glutamate almost inactive Pseudomonas aeruginosa ?
-
?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 9
-
Pseudomonas aeruginosa