Literature summary for 2.7.2.3 extracted from

Piper, P.W.; Emson, C.; Jones, C.E.; Cowan, D.A.; Fleming, T.M.; Littlechild, J.A.
Complementation of a pgk deletion mutation in Saccharomyces cerevisiae with expression of the phosphoglycerate-kinase gene from the hyperthermophilic archaeon Sulfolobus solfataricus (1996), Curr. Genet., 29, 594-596.

Cloned(Commentary)

Cloned (Comment)	Organism
the gene encoding phosphoglycerate kinase is inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector is then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants are obtained on galactose plates at 37°C, but not at 28°C. These transformants contain low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enables complementation of the yeast pgk defect at 37°C but not at 28°C	Saccharolobus solfataricus

Cloned (Comment)

Organism

the gene encoding phosphoglycerate kinase is inserted into a yeast expression vector under the control of the galactose-inducible GAL1 yeast promoter. This vector is then transformed into a pgk::TRP1 yeast mutant, a strain inhibited for growth on galactose or glucose due to its lack of PGK enzyme. Slow-growing transformants are obtained on galactose plates at 37°C, but not at 28°C. These transformants contain low levels of transcripts of the heterologous gene and low amounts of thermostable PGK activity. Weak expression of the hyperthermophile gene in yeast, a mesophile, therefore enables complementation of the yeast pgk defect at 37°C but not at 28°C

Saccharolobus solfataricus

Organism

Organism	UniProt	Comment	Textmining
Saccharolobus solfataricus	-	-	-

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Release 2023.1 (January 2023)