Cloned (Comment) | Organism |
---|---|
mutant W290Y | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
W290Y | has a negligible effect on its catalytic activity but improves protein solubility | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | P18912 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant W290Y | Geobacillus stearothermophilus |
Storage Stability | Organism |
---|---|
4°C, 80% saturated ammonium sulfate solution | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + 3-phospho-D-glyceroyl 1-phosphate | overall increases in amide protection from hydrogen exchange when the W290Y mutant protein binds the substrate and product ligands and an additional increase when the transition-state analogue complex containing a 3-phospho-D-glycerate-(AlF4-)-ADP moiety is formed. Communication between domains is manifested in the accessibility of higher-energy, exchange-competent states. For residue D33, chemical shifts only change significantly upon transition-state analogue binding. For residue S346, chemical shifts change significantly upon ADP and transition-state analogue binding. For residue S58, chemical shifts are affected by 3-phospho-D-glycerate and transition-state analogue binding | Geobacillus stearothermophilus | ATP + 3-phospho-D-glycerate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PGK | - |
Geobacillus stearothermophilus |
phosphoglycerate kinase | - |
Geobacillus stearothermophilus |