Activating Compound | Comment | Organism | Structure |
---|---|---|---|
acetate | as Na or K salt continues to activate at concentrations above 200 mM | Plasmodium falciparum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
hanging-drop vapour-diffusion method, recombinant enzyme forms a hexagonal space group P6(1)22 with unit cell parameters a = b = 140.7 A, c = 263.9 A, 3.0 A resolution | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KH2PO4 | above 50 mM | Plasmodium falciparum | |
NaH2PO4 | above 50 mM | Plasmodium falciparum | |
suramin | IC50: 0.007 mM | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.52 | - |
3-phospho-D-glycerate | - |
Plasmodium falciparum | |
0.63 | - |
ATP | - |
Plasmodium falciparum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation | Plasmodium falciparum | |
KCl | maximal activation at 100 mM | Plasmodium falciparum | |
KH2PO4 | maximal activation at 25-50 mM | Plasmodium falciparum | |
Mg2+ | divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation. Optimal concentration of Mg2+ is 5-15 mM | Plasmodium falciparum | |
Mn2+ | divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation | Plasmodium falciparum | |
NaCl | maximal activation at 100 mM | Plasmodium falciparum | |
NaH2PO4 | maximal activation at 25-50 mM | Plasmodium falciparum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
x * 45000, SDS-PAGE | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Plasmodium falciparum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
210 | - |
- |
Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Plasmodium falciparum | ADP + 3-phospho-D-glyceroyl phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 45000, SDS-PAGE | Plasmodium falciparum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
30 min, 27% loss of activity | Plasmodium falciparum |
37 | - |
30 min, nearly 80% loss of activity | Plasmodium falciparum |
49 | - |
30 min, approximately 95% loss of activity | Plasmodium falciparum |
61 | - |
30 min, enzyme retains less than 4% of its activity | Plasmodium falciparum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 7.9 | - |
Plasmodium falciparum |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.007 | - |
IC50: 0.007 mM | Plasmodium falciparum | suramin |