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Literature summary for 2.7.2.3 extracted from

  • Pal, B.; Pybus, B.; Muccio, D.D.; Chattopadhyay, D.
    Biochemical characterization and crystallization of recombinant 3-phosphoglycerate kinase of Plasmodium falciparum (2004), Biochim. Biophys. Acta, 1699, 277-280.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
acetate as Na or K salt continues to activate at concentrations above 200 mM Plasmodium falciparum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method, recombinant enzyme forms a hexagonal space group P6(1)22 with unit cell parameters a = b = 140.7 A, c = 263.9 A, 3.0 A resolution Plasmodium falciparum

Inhibitors

Inhibitors Comment Organism Structure
KH2PO4 above 50 mM Plasmodium falciparum
NaH2PO4 above 50 mM Plasmodium falciparum
suramin IC50: 0.007 mM Plasmodium falciparum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.52
-
3-phospho-D-glycerate
-
Plasmodium falciparum
0.63
-
ATP
-
Plasmodium falciparum

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation Plasmodium falciparum
KCl maximal activation at 100 mM Plasmodium falciparum
KH2PO4 maximal activation at 25-50 mM Plasmodium falciparum
Mg2+ divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation. Optimal concentration of Mg2+ is 5-15 mM Plasmodium falciparum
Mn2+ divalent metal ion required: Ca2+, Mg2+ or Mn2+. Mg2+ is the preferred cation Plasmodium falciparum
NaCl maximal activation at 100 mM Plasmodium falciparum
NaH2PO4 maximal activation at 25-50 mM Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
x * 45000, SDS-PAGE Plasmodium falciparum

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Plasmodium falciparum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
210
-
-
Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 3-phospho-D-glycerate
-
Plasmodium falciparum ADP + 3-phospho-D-glyceroyl phosphate
-
?

Subunits

Subunits Comment Organism
? x * 45000, SDS-PAGE Plasmodium falciparum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
30 min, 27% loss of activity Plasmodium falciparum
37
-
30 min, nearly 80% loss of activity Plasmodium falciparum
49
-
30 min, approximately 95% loss of activity Plasmodium falciparum
61
-
30 min, enzyme retains less than 4% of its activity Plasmodium falciparum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 7.9
-
Plasmodium falciparum

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.007
-
IC50: 0.007 mM Plasmodium falciparum suramin