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Literature summary for 2.7.2.3 extracted from
- Human erythrocyte phosphoglycerate kinase: purification, properties, and interaction with its antibody (1973), Biochim. Biophys. Acta, 321, 503-511.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization via precipitation with ammonium sulfate stepwise from 60% to 75% w/v at room temperature for 2 h, then 4°C, several days | Homo sapiens |
General Stability
| General Stability | Organism |
|---|---|
| crystalline enzyme is rather unstable, dried crystals or dilute aqueous solutions lose all activity within a few weeks | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | partial inhibition in presence of Mg2+ | Homo sapiens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partially replace Mg2+ in activation | Homo sapiens | |
| Ca2+ | 30% of the activity with Mg2+ | Homo sapiens | |
| divalent cation | requires Mg2+ or Ca2+ | Homo sapiens | |
| Mg2+ | required | Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| erythrocyte | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 580 | - |
purified crystalline enzyme | Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, crystals as a suspension in 3 M (NH4)2SO4, 1 mM sodium diphosphate, 1 mM EDTA | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | absolute specificity for 3-phospho-D-glycerate | Homo sapiens | ADP + 1,3-diphosphoglycerate | - |
r | |
| GTP + 3-phospho-D-glycerate | 60% of the activity with ATP | Homo sapiens | GDP + 1,3-diphosphoglycerate | - |
? | |
| ITP + 3-phospho-D-glycerate | 64% of the activity with ATP | Homo sapiens | IDP + 1,3-diphosphoglycerate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Homo sapiens | |
| ATP | required as phosphate donor | Homo sapiens | |
| GTP | - |
Homo sapiens | |
| ITP | - |
Homo sapiens | |
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Release 2023.1 (January 2023)
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