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Literature summary for 2.7.2.3 extracted from
- Liver 3-phosphoglycerate kinase. Purification and some molecular properties of the bovine-liver enzyme (1974), Eur. J. Biochem., 45, 321-331.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| crystallization from ammonium sulfate precipitation | Bos taurus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 250fold from liver, to homogeneity | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 143 | - |
purified enzyme | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Bos taurus | ADP + 1,3-diphosphoglycerate | - |
r |  |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
unstable below | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Bos taurus | |
| ATP | required as phosphate donor | Bos taurus | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Bos taurus | 3 peaks of purified enzyme at pH 3.8, pH 5.75, and pH 7.45, isoelectric focusing using an analytical column with carrier ampholytes | - |
3.8 |
| Bos taurus | 3 peaks of purified enzyme at pH 3.8, pH 5.75, and pH 7.45, isoelectric focusing using an analytical column with carrier ampholytes | - |
5.75 |
| Bos taurus | 3 peaks of purified enzyme at pH 3.8, pH 5.75, and pH 7.45, isoelectric focusing using an analytical column with carrier ampholytes | - |
7.45 |
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Release 2023.1 (January 2023)
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