Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Co2+ | 58% | Geobacillus stearothermophilus | |
nucleoside diphosphates | purine nucleotide diphosphates | Geobacillus stearothermophilus | |
nucleoside monophosphates | purine nucleotide monophosphates | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.2 | - |
3-phospho-D-glycerate | pH 7.6, 25°C | Geobacillus stearothermophilus | |
2.9 | - |
ATP | pH 7.6, 25°C | Geobacillus stearothermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
Ca2+ | 15% of the activity with Mg2+ | Geobacillus stearothermophilus | |
Co2+ | can partially replace Mg2+ in activation | Geobacillus stearothermophilus | |
Co2+ | 58% of the activity with Mg2+ | Geobacillus stearothermophilus | |
divalent cation | Mg2+ or Mn2+ | Geobacillus stearothermophilus | |
Mg2+ | - |
Geobacillus stearothermophilus | |
Mn2+ | Mn2+ or Mg2+ required | Geobacillus stearothermophilus | |
additional information | no activation by Ba2+ | Geobacillus stearothermophilus | |
Ni2+ | no activation | Geobacillus stearothermophilus | |
Zn2+ | no activation | Geobacillus stearothermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
gel filtration | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | - |
NCA 1503 | - |
Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
Purification (Comment) | Organism |
---|---|
- |
Geobacillus stearothermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
557 | - |
purified enzyme | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | absolute specificity for 3-phospho-D-glycerate | Geobacillus stearothermophilus | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | absolute specificity for 3-phospho-D-glycerate | Geobacillus stearothermophilus NCA 1503 | ADP + 1,3-diphosphoglycerate | - |
r | |
GTP + 3-phospho-D-glycerate | 27% of the activity with ATP | Geobacillus stearothermophilus | GDP + 1,3-diphosphoglycerate | - |
? | |
GTP + 3-phospho-D-glycerate | 27% of the activity with ATP | Geobacillus stearothermophilus NCA 1503 | GDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | 42% of the activity with ATP | Geobacillus stearothermophilus | IDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | 42% of the activity with ATP | Geobacillus stearothermophilus NCA 1503 | IDP + 1,3-diphosphoglycerate | - |
? | |
additional information | no activity with pyrimidine nucleotides | Geobacillus stearothermophilus | ? | - |
? | |
additional information | no activity with pyrimidine nucleotides | Geobacillus stearothermophilus NCA 1503 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 42000, SDS-PAGE | Geobacillus stearothermophilus |
More | secondary structure: 20% alpha-helix, 45% beta-structure | Geobacillus stearothermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Geobacillus stearothermophilus |
55 | 60 | physiological temperature | Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
10 min, stable | Geobacillus stearothermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | 8.5 | - |
Geobacillus stearothermophilus |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
inactivation below | Geobacillus stearothermophilus |
6 | - |
stable above | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Geobacillus stearothermophilus | |
ATP | true substrate is the magnesium complexes of ATP | Geobacillus stearothermophilus | |
ATP | required as phosphate donor | Geobacillus stearothermophilus | |
GTP | - |
Geobacillus stearothermophilus | |
ITP | - |
Geobacillus stearothermophilus |