Activating Compound | Comment | Organism | Structure |
---|---|---|---|
SO42- | activation with yeast PGK, inhibition with Trypanosomal PGK | Trypanosoma brucei |
Cloned (Comment) | Organism |
---|---|
gene PGKA, expression in Escherichia coli deficient mutant as a fusion protein, complementation of the mutant phenotype | Trypanosoma brucei |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
SO42- | activation with yeast PGK, inhibition with trypanosomal PGK | Trypanosoma brucei |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
ATP | recombinant isozyme 56PGK | Trypanosoma brucei | |
0.12 | - |
ATP | ATP in form of MgATP2- | Trypanosoma brucei | |
2.4 | - |
3-phospho-D-glycerate | recombinant isozyme 56PGK | Trypanosoma brucei |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
glycosome | isozyme 56PGK, glycosomal microbody | Trypanosoma brucei | 20015 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Trypanosoma brucei | - |
glycosomal isozyme 56PGK, gene PGKA | - |
Trypanosoma brucei | - |
cytoplasmic isozyme | - |
Purification (Comment) | Organism |
---|---|
recombinant from Escherichia coli, to homogeneity | Trypanosoma brucei |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 3-phospho-D-glycerate | - |
Trypanosoma brucei | ADP + 1,3-diphosphoglycerate | - |
r | |
ATP + 3-phospho-D-glycerate | - |
Trypanosoma brucei | ADP + 3-phospho-D-glyceroyl phosphate | - |
r | |
GTP + 3-phospho-D-glycerate | no activity | Trypanosoma brucei | GDP + 1,3-diphosphoglycerate | - |
? | |
ITP + 3-phospho-D-glycerate | no activity | Trypanosoma brucei | IDP + 1,3-diphosphoglycerate | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Trypanosoma brucei | |
ATP | required as phosphate donor | Trypanosoma brucei |