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Literature summary for 2.7.2.3 extracted from
- Simultaneous purification of hexokinase, class-I fructose-bisphosphate aldolase, triosephosphate isomerase and phosphoglycerate kinase from Trypanosoma brucei (1984), Eur. J. Biochem., 144, 475-483.
General Stability
| General Stability | Organism |
|---|---|
| extensive dialysis against 0.1 M Tris/HCl, pH 7.6, 1 mM DTT, 1 mM EDTA, 1 mM sodium azide, 0.2 M ammonium sulfate, stable | Trypanosoma brucei |
| freezing inactivates | Trypanosoma brucei |
| stable as a suspension in ammonium sulfate, 70-90% saturation | Trypanosoma brucei |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| glycosome | - |
Trypanosoma brucei | 20015 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Trypanosoma brucei |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
x * 46000, SDS-PAGE | Trypanosoma brucei |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trypanosoma brucei | - |
bloodstream form, stock 427 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 660fold, to homogeneity | Trypanosoma brucei |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 504 | - |
purified enzyme | Trypanosoma brucei |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 3-phospho-D-glycerate | - |
Trypanosoma brucei | ADP + 1,3-diphosphoglycerate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46000, SDS-PAGE | Trypanosoma brucei |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Trypanosoma brucei |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.6 | - |
assay at | Trypanosoma brucei |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ADP | - |
Trypanosoma brucei | |
| ATP | required as phosphate donor | Trypanosoma brucei | |
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Release 2023.1 (January 2023)
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