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Literature summary for 2.7.2.14 extracted from

  • Harwood, C.S.; Canale-Parola, E.
    Properties of acetate kinase isozymes and a branched-chain fatty acid kinase from a spirochete (1982), J. Bacteriol., 152, 246-254.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.2.14

General Stability

General Stability Organism
complete loss of activity if filtered through columns of Sephacryl S-300 at room temperature Spirochaeta sp.
dialysis, 6 h at 5°C, significant loss of activity Spirochaeta sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8
-
 ATP pH 7.5, 30°C Spirochaeta sp.
4.3
-
 Isobutyrate pH 7.5, 30°C Spirochaeta sp.
9.5
-
 Isovalerate pH 7.5, 30°C Spirochaeta sp.
10.8
-
 2-methylbutyrate pH 7.5, 30°C Spirochaeta sp.
12.5
-
 valerate pH 7.5, 30°C Spirochaeta sp.
14.3
-
 propionate pH 7.5, 30°C Spirochaeta sp.
16.9
-
 Butyrate pH 7.5, 30°C Spirochaeta sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 65% of the activity with Mn2+ Spirochaeta sp.
Cu2+ 58% of the activity with Mn2+ Spirochaeta sp.
Mg2+ 96% of the activity with Mn2+ Spirochaeta sp.
Mn2+ divalent cation required, highest activity with Mn2+ Spirochaeta sp.
Zn2+ 42% of the activity with Mn2+ Spirochaeta sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
76000
-
 gel filtration Spirochaeta sp.

Organism

Organism UniProt Comment Textmining
Spirochaeta sp.
-
 MA-2
-
Spirochaeta sp. MA-2
-
 MA-2
-

Storage Stability

Storage Stability Organism
-50°C, crude cell extract stable for 2 months Spirochaeta sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 2-methylbutanoate
-
 Spirochaeta sp. ADP + 2-methylbutanoyl phosphate
-
 ?
ATP + butanoate
-
 Spirochaeta sp. ADP + butanoyl phosphate
-
 ?
ATP + isobutanoate
-
 Spirochaeta sp. ADP + isobutanoyl phosphate
-
 ?
ATP + isopentanoate
-
 Spirochaeta sp. ADP + isopentanoyl phosphate
-
 ?
ATP + pentanoate
-
 Spirochaeta sp. ADP + pentanoyl phosphate
-
 ?
ATP + propionate
-
 Spirochaeta sp. ADP + propanoyl phosphate
-
 ?
CTP + isobutanoate 103% of the activity with ATP Spirochaeta sp. CDP + isobutanoyl phosphate
-
 ?
GTP + isobutanoate 70% of the activity with ATP Spirochaeta sp. GDP + isobutanoyl phosphate
-
 ?
ITP + isobutanoate 68% of the activity with ATP Spirochaeta sp. IDP + isobutanoyl phosphate
-
 ?
additional information no actrivity with acetate Spirochaeta sp. ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 30 min, 25% loss of activity Spirochaeta sp.
60
-
 30 min, complete loss of activity Spirochaeta sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
-
 Spirochaeta sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 8.5 more than 80% of maximal activity at pH 6.0 and 8.5 Spirochaeta sp.