Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a His-tagged fusion protein | Thermotoga maritima |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | - |
Thermotoga maritima | |
cis-4-hydroxy-L-proline | - |
Thermotoga maritima | |
methyl L-proline | - |
Thermotoga maritima | |
trans-4-hydroxy-L-proline | - |
Thermotoga maritima |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
SDS-PAGE | Thermotoga maritima |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermotoga maritima | - |
- |
- |
Purification (Comment) | Organism |
---|---|
(NH4)2SO4 precipitation and a single ion-exchange chromatography step are used | Thermotoga maritima |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate | - |
Thermotoga maritima | ADP + L-glutamate 5-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | gel filtration, 4 * 42000 | Thermotoga maritima |
Synonyms | Comment | Organism |
---|---|---|
glutamate kinase | - |
Thermotoga maritima |
proB | - |
Thermotoga maritima |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
83 | - |
- |
Thermotoga maritima |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 85 | the enzyme retains approximately 100, 90 and 65% of its activity when heated for 45 min at 70, 80 and 85°C, respectively | Thermotoga maritima |
90 | - |
at 90°C, tmGK displays around 40% of the activity when heated for 45 min | Thermotoga maritima |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9 | - |
Thermotoga maritima |