Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | GK activity requires gamma-glutamyl phosphate reductase | Saccharomyces cerevisiae |
Application | Comment | Organism |
---|---|---|
additional information | GK is the rate-limiting enzyme in the proline biosynthesis | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of PRO1 in Escherichia coli M15(pREP4) | Saccharomyces cerevisiae |
Protein Variants | Comment | Organism |
---|---|---|
D154N | is 64fold less sensitive to feedback inhibition than wild-type GK, leading to proline accumulation | Saccharomyces cerevisiae |
E149K | very insensitive to feedback inhibition, shows prominent increase in the proline content, freeze tolerance like the D154N mutant | Saccharomyces cerevisiae |
I150T | very insensitive to feedback inhibition, shows prominent increase in the proline content, is more tolerant to freezing stress than cells expressing the D154N mutant | Saccharomyces cerevisiae |
N142D/I166V | very insensitive to feedback inhibition, shows prominent increase in the proline content, is more tolerant to freezing stress than cells expressing the D154N mutant | Saccharomyces cerevisiae |
S146P | shows prominent increase in the proline content | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
L-proline | GK activity is subject to feedback inhibition by proline | Saccharomyces cerevisiae | |
additional information | no significant differences in growth between mutant yeast cells on SD agar medium with heat shock (50°C, 3 to 5 h), ethanol (16 to20%), and sorbitol (2.0 to 2.5 M) stresses, although the proline-accumulating cells are more resistant to these stresses than cells expressing wild-type GK | Saccharomyces cerevisiae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtration | Saccharomyces cerevisiae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P32264 | strain INVDput1pro1 | - |
Purification (Comment) | Organism |
---|---|
by Ni2+ column and gel filtration | Saccharomyces cerevisiae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
mutant N142D/I166V | Saccharomyces cerevisiae |
4.2 | - |
mutant E149K | Saccharomyces cerevisiae |
14.7 | - |
wild-type | Saccharomyces cerevisiae |
26.3 | - |
mutant D154N | Saccharomyces cerevisiae |
29 | - |
mutant I150T | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + L-glutamate | - |
Saccharomyces cerevisiae | ADP + L-glutamate 5-phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
gamma-glutamyl kinase | - |
Saccharomyces cerevisiae |
GK | - |
Saccharomyces cerevisiae |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.5 | - |
wild-type | Saccharomyces cerevisiae | L-proline | |
32 | - |
mutant D154N | Saccharomyces cerevisiae | L-proline | |
50 | - |
mutant I150T | Saccharomyces cerevisiae | L-proline | |
54 | - |
mutant N142D/I166V | Saccharomyces cerevisiae | L-proline | |
840 | - |
mutant E149K | Saccharomyces cerevisiae | L-proline |