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Literature summary for 2.7.2.1 extracted from

  • Singh-Wissmann, K.; Miles, R.D.; Ingram-Smith, C.; Ferry, J.G.
    Identification of essential arginines in the acetate kinase from Methanosarcina thermophila (2000), Biochemistry, 39, 3671-3677.
    View publication on PubMed
Search for more literature for 2.7.2.1

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant acetate kinase genes subcloned in to the T7-based expression vector pET15b Methanosarcina thermophila

Protein Variants

Protein Variants Comment Organism
R175K site-directed mutagenesis Methanosarcina thermophila
R285A site-directed mutagenesis Methanosarcina thermophila
R285K site-directed mutagenesis Methanosarcina thermophila
R285L site-directed mutagenesis Methanosarcina thermophila
R340K site-directed mutagenesis Methanosarcina thermophila
R340L site-directed mutagenesis Methanosarcina thermophila
R91K site-directed mutagenesis Methanosarcina thermophila

Inhibitors

Inhibitors Comment Organism Structure
Phenylglyoxal
-
 Methanosarcina thermophila

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.9
-
 ATP pH 7.6, 37°C, mutant R340L Methanosarcina thermophila
1
-
 ATP pH 7.6, 37°C, wild-type and mutant R175K Methanosarcina thermophila
1.3
-
 ATP pH 7.6, 37°C, mutant R340K Methanosarcina thermophila
1.4
-
 ATP
-
 Methanosarcina thermophila
2.3
-
 acetate
-
 Methanosarcina thermophila
2.8
-
 ATP pH 7.6, 37°C, recombinant wild-type Methanosarcina thermophila
3
-
 ATP pH 7.6, 37°C, mutant R285K, R285L Methanosarcina thermophila
3.4
-
 ATP pH 7.6, 37°C, mutant R241K Methanosarcina thermophila
3.9
-
 ATP pH 7.6, 37°C, mutant R91K Methanosarcina thermophila
4
-
 acetate pH 7.6, 37°C, mutant R175K Methanosarcina thermophila
6
-
 ATP pH 7.6, 37°C, mutant R285A, R91A Methanosarcina thermophila
7.2
-
 acetate
-
 Methanosarcina thermophila
19
-
 acetate pH 7.6, 37°C Methanosarcina thermophila
20
-
 acetate pH 7.6, 37°C, mutant R340L Methanosarcina thermophila
42
-
 acetate pH 7.6, 37°C, mutant R340K Methanosarcina thermophila
129
-
 acetate pH 7.6, 37°C, mutant R285L Methanosarcina thermophila
206
-
 acetate pH 7.6, 37°C, mutant R285A Methanosarcina thermophila
219
-
 acetate pH 7.6, 37°C, mutant R285K Methanosarcina thermophila
260
-
 acetate pH 7.6, 37°C, mutant R91K Methanosarcina thermophila

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + acetate Methanosarcina thermophila
-
 ADP + acetyl phosphate
-
 r

Organism

Organism UniProt Comment Textmining
Methanosarcina thermophila
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified wild-type and recombinant enzymes, R175L and R340A are unstable and cannot be purified Methanosarcina thermophila

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 r