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Literature summary for 2.7.2.1 extracted from
- Purification and characterization of acetate kinase from Clostridium thermocellum (1998), Tetrahedron, 54, 15915-15925.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| industry | enzyme involved in the wasteful production of acetate during conversion of cellulose to ethanol | Acetivibrio thermocellus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 135 | - |
acetate | pH 7.2-8.0, 50°C | Acetivibrio thermocellus |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Acetivibrio thermocellus | |
| Mn2+ | - |
Acetivibrio thermocellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 46000 | - |
2 * 46000, homodimer, SDS-PAGE | Acetivibrio thermocellus |
| 84000 | - |
gel filtration | Acetivibrio thermocellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Acetivibrio thermocellus | - |
ADP + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
ATCC 27405 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acetivibrio thermocellus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 490 | - |
- |
Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | - |
Acetivibrio thermocellus | ADP + acetyl phosphate | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 46000, homodimer, SDS-PAGE | Acetivibrio thermocellus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Acetivibrio thermocellus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | - |
Acetivibrio thermocellus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | 80 | retains full activity, stable up to 60°C, loses 55% of its activity at 70°C, completely inactivated after 2 h at 80°C | Acetivibrio thermocellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | 8 | - |
Acetivibrio thermocellus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | - |
Acetivibrio thermocellus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | quite stable between pH 7.0 to 9.0, only 15% loss in activity at pH 10.0, inactivated rapidly outside this range | Acetivibrio thermocellus |
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