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Literature summary for 2.7.2.1 extracted from
- Acetate kinase from Bacillus stearothermophilus (1982), Methods Enzymol., 90, 179-185.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| alpha-glycerophosphate | - |
Geobacillus stearothermophilus |  |
| D-fructose 1,6-bisphosphate | activates formation of ADP | Geobacillus stearothermophilus | |
| D-Glucose 1,6-bisphosphate | - |
Geobacillus stearothermophilus | |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Geobacillus stearothermophilus | |
| Mn2+ | - |
Geobacillus stearothermophilus | |
| additional information | Co2+, Ca2+, Cd2+ and Zn2+ can replace Mg2+ or Mn2+ only partially | Geobacillus stearothermophilus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
| 160000 | 170000 | gel filtration | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | Geobacillus stearothermophilus | function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | Geobacillus stearothermophilus NCA 1503 | function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase | ADP + acetyl phosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Geobacillus stearothermophilus | - |
NCA 1503 | - |
| Geobacillus stearothermophilus NCA 1503 | - |
NCA 1503 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Geobacillus stearothermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1530 | - |
- |
Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acetate | - |
Geobacillus stearothermophilus | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase | Geobacillus stearothermophilus | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | - |
Geobacillus stearothermophilus NCA 1503 | ADP + acetyl phosphate | - |
r | |
| ATP + acetate | function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase | Geobacillus stearothermophilus NCA 1503 | ADP + acetyl phosphate | - |
r | |
| ATP + propionate | poor acceptor, 5% relative to acetate at 500 mM | Geobacillus stearothermophilus | ADP + propionyl phosphate | - |
r | |
| ATP + propionate | poor acceptor, 5% relative to acetate at 500 mM | Geobacillus stearothermophilus NCA 1503 | ADP + propionyl phosphate | - |
r | |
| GTP + acetate | - |
Geobacillus stearothermophilus | GDP + acetyl phosphate | - |
r | |
| GTP + acetate | - |
Geobacillus stearothermophilus NCA 1503 | GDP + acetyl phosphate | - |
r | |
| additional information | UTP and CTP are active only partially | Geobacillus stearothermophilus | ? | - |
? | |
| additional information | UTP and CTP are active only partially | Geobacillus stearothermophilus NCA 1503 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Geobacillus stearothermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
quite heat stable | Geobacillus stearothermophilus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.3 | - |
- |
Geobacillus stearothermophilus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 8 | stable, rapid inactivation at pH values lower than 6.0, rather stable at alkaline pH | Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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