Literature summary for 2.7.2.1 extracted from

Bock, A.K.; Glasemacher, J.; Schmidt, R.; Schonheit, P.
Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritima (1999), J. Bacteriol., 181, 1861-1867.

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General Stability

General Stability	Organism
stabilized against heat inactivation by salts, most effective (NH4)2SO4	Thermotoga maritima

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.44	-	acetyl phosphate	pH 7.0, 50°C	Thermotoga maritima
0.7	-	ATP	pH 7.0, 50°C	Thermotoga maritima
3	-	ADP	pH 7.0, 50°C	Thermotoga maritima
40	-	acetate	pH 7.0, 50°C	Thermotoga maritima

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	-	Thermotoga maritima
Co2+	-	Thermotoga maritima
Mg2+	-	Thermotoga maritima
Mn2+	-	Thermotoga maritima
Ni2+	-	Thermotoga maritima
Zn2+	-	Thermotoga maritima

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	2 * 44000, alpha2 homodimer, SDS-PAGE	Thermotoga maritima
90000	-	gel filtration	Thermotoga maritima

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
ATP + acetate	Thermotoga maritima	-	ADP + acetyl phosphate	-	r

Organism

Organism	UniProt	Comment	Textmining
Thermotoga maritima	-	-	-

Oxidation Stability

Oxidation Stability	Organism
not sensitive to oxygen	Thermotoga maritima

Purification (Commentary)

Purification (Comment)	Organism
-	Thermotoga maritima

Storage Stability

Storage Stability	Organism
-20°C, can be stored in 20 mM Tris-HCl, pH 8.0, 2.0 mM MgCl2, 0.32 M NaCl, supplemented with 10% glycerol, without significant loss of activity for several weeks	Thermotoga maritima

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + acetate	-	Thermotoga maritima	ADP + acetyl phosphate	-	r
ATP + propionate	-	Thermotoga maritima	ADP + propionyl phosphate	-	r
CTP + acetate	-	Thermotoga maritima	CDP + acetyl phosphate	-	r
GTP + acetate	-	Thermotoga maritima	GDP + acetyl phosphate	-	r
ITP + acetate	-	Thermotoga maritima	IDP + acetyl phosphate	-	r
additional information	butyrate, isobutyrate, valerate and isovalerate are no substrates	Thermotoga maritima	?	-	?
UTP + acetate	-	Thermotoga maritima	UDP + acetyl phosphate	-	r

Subunits

Subunits	Comment	Organism
dimer	2 * 44000, alpha2 homodimer, SDS-PAGE	Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
90	-	-	Thermotoga maritima

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
40	100	activity increases rapidly above 55°C, enzyme does not lose activity at 80°C upon incubation for 180 min	Thermotoga maritima

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
55	100	almost complete loss about 90% is observed after incubation at 100°C for about 60 min, enzyme does not lose activity upon incubation for 3 h at 100°C in presence of 1 M (NH4)2SO4	Thermotoga maritima

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Thermotoga maritima

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	8.5	about 50% activity in this range	Thermotoga maritima

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Release 2023.1 (January 2023)