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Literature summary for 2.7.11.12 extracted from
- Expression of constitutively active cGMP-dependent protein kinase inhibits glucose-induced vascular smooth muscle cell proliferation (2009), Am. J. Physiol. Heart Circ. Physiol., 297, H2075-H2083.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cGMP | - |
Mus musculus |  |
| cGMP | - |
Homo sapiens | |
| cGMP | - |
Rattus norvegicus | |
| cGMP | - |
Bos taurus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| the cDNA of bovine catalytic domain of PKG Ialpha, PKG-CD, is inserted in expression vector of pcDNA4/TO/myc-His C/CD | Bos taurus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
catalytic domain of PKG Ialpha, PKG-CD, His-tagged, determined by SDS-PAGE and Western blot analysis | Bos taurus |
| 77000 | - |
endogenous PKG-I, determined by SDS-PAGE and Western blot analysis | Mus musculus |
| 77000 | - |
endogenous PKG-I, determined by SDS-PAGE and Western blot analysis | Homo sapiens |
| 77000 | - |
endogenous PKG-I, determined by SDS-PAGE and Western blot analysis | Rattus norvegicus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | Mus musculus | - |
ADP + a phosphoprotein | - |
? | |
| ATP + a protein | Homo sapiens | - |
ADP + a phosphoprotein | - |
? | |
| ATP + a protein | Rattus norvegicus | - |
ADP + a phosphoprotein | - |
? | |
| ATP + a protein | Bos taurus | - |
ADP + a phosphoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| abdominal aorta | - |
Mus musculus | - |
| abdominal aorta | - |
Rattus norvegicus | - |
| aortic smooth muscle cell | - |
Homo sapiens | - |
| thoracic aorta | - |
Mus musculus | - |
| thoracic aorta | - |
Rattus norvegicus | - |
| vascular smooth muscle cell | VCSM | Mus musculus | - |
| vascular smooth muscle cell | VCSM | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | - |
Mus musculus | ADP + a phosphoprotein | - |
? | |
| ATP + a protein | - |
Homo sapiens | ADP + a phosphoprotein | - |
? | |
| ATP + a protein | - |
Rattus norvegicus | ADP + a phosphoprotein | - |
? | |
| ATP + a protein | - |
Bos taurus | ADP + a phosphoprotein | - |
? | |
| ATP + RKISASEFDRPL | BPDEtide, substrate in activity assay | Bos taurus | ADP + phosphorylated RKISASEFDRPL | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cGMP-dependent protein kinase | - |
Mus musculus |
| cGMP-dependent protein kinase | - |
Homo sapiens |
| cGMP-dependent protein kinase | - |
Rattus norvegicus |
| cGMP-dependent protein kinase | - |
Bos taurus |
| PKG | - |
Mus musculus |
| PKG | - |
Homo sapiens |
| PKG | - |
Rattus norvegicus |
| PKG | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
activity assay | Bos taurus |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Mus musculus | cGMP-dependent protein kinase is downregulated in vessels from diabetic animals or in vascular smooth muscle cells exposed to high-glucose conditions | down |
| Homo sapiens | cGMP-dependent protein kinase is downregulated in vessels from diabetic animals or in vascular smooth muscle cells exposed to high-glucose conditions | down |
| Rattus norvegicus | cGMP-dependent protein kinase is downregulated in vessels from diabetic animals or in vascular smooth muscle cells exposed to high-glucose conditions | down |
| Homo sapiens | cGMP-dependent protein kinase activity is restored in vascular smooth muscle cells by transfection of primary human aortic smooth muscle cells with an expression vector coding for the catalytic domain of bovine PKG | up |
| Mus musculus | cGMP-dependent protein kinase activity is restored in vascular smooth muscle cells by transfection of primary mouse aortic smooth muscle cells with an expression vector coding for the catalytic domain of bovine PKG | up |
| Rattus norvegicus | cGMP-dependent protein kinase activity is restored in vascular smooth muscle cells by transfection of primary rat aortic smooth muscle cells with an expression vector coding for the catalytic domain of bovine PKG | up |
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