Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | dependent on | Mus musculus | |
cAMP | dependent on | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of His-tagged catalytic subunit Calpha in Escherichia coli strain BL21(DE3), expression of catalytic subunit Calpha in Spodoptera frugiperda Sf9 cells via baculovirus infection system | Mus musculus |
expression of His-tagged catalytic subunit Cgamma in Escherichia coli strain BL21(DE3), stable expression of catalytic subunit Cgamma in cAMP-resistant mouse Y1 adrenal mutant cell line, expression of catalytic subunit Cgamma in Spodoptera frugiperda Sf9 cells via baculovirus infection system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | no inhibition of catalytic subunit Cgamma by PKI | Homo sapiens | |
PKI | catalytic subunit Calpha | Mus musculus | |
pseudosubstrate peptides | IC50 values for inhibition of the regulatory subunits of PKA by endogenous inhibitors, overview | Homo sapiens | |
pseudosubstrate peptides | IC50 values for inhibition of he regulatory subunits of PKA by endogenous inhibitors, overview | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics for catalytic subunit Cgamma | Mus musculus | |
additional information | - |
additional information | kinetics for catalytic subunit Cgamma | Homo sapiens | |
0.0125 | - |
Kemptide | pH 7.0, 30°C, recombinant catalytic subunit Cgamma | Homo sapiens | |
0.0253 | - |
RFARKGSLREKNV | pH 7.0, 30°C, recombinant catalytic subunit Cgamma | Homo sapiens | |
0.0333 | - |
RKRSRAE | pH 7.0, 30°C, recombinant catalytic subunit Cgamma | Homo sapiens | |
0.0333 | - |
RKRSRKE | pH 7.0, 30°C, recombinant catalytic subunit Cgamma | Homo sapiens | |
0.038 | - |
Kemptide | pH 7.0, 30°C, recombinant catalytic subunit Calpha | Mus musculus | |
0.05 | - |
RFARKGSLREKNV | pH 7.0, 30°C, recombinant catalytic subunit Calpha | Mus musculus | |
0.0503 | - |
RRLSSLRA | pH 7.0, 30°C, recombinant catalytic subunit Cgamma | Homo sapiens | |
0.293 | - |
RKRSRAE | pH 7.0, 30°C, recombinant catalytic subunit Calpha | Mus musculus | |
0.338 | - |
RRLSSLRA | pH 7.0, 30°C, recombinant catalytic subunit Calpha | Mus musculus | |
0.5 | - |
RKRSRKE | pH 7.0, 30°C, recombinant catalytic subunit Calpha | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Mus musculus | |
Mg2+ | - |
Homo sapiens | |
Zn2+ | - |
Mus musculus | |
Zn2+ | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
1 * 41000, isolated recombinant catalytic subunit Cgamma from Sf9 cells, SDS-PAGE | Homo sapiens |
42000 | - |
1 * 42000, isolated recombinant catalytic subunit Calpha from Escherichia coli, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant catalytic subunit Calpha from Escherichia coli by nickel affinity chromatography, recombinant catalytic subunit Calpha from Sf9 insect cells 62fold by adsorption chromatography and gel filtration | Mus musculus |
recombinant His-tagged catalytic subunit Cgamma from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant catalytic subunit Cgamma 273fold from Sf9 insect cells by adsorption chromatography and gel filtration | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.31 | - |
purified recombinant catalytic subunit Cgamma, substrate Kemptide | Homo sapiens |
0.81 | - |
purified recombinant catalytic subunit Cgamma, substrate histone | Homo sapiens |
5.3 | - |
purified recombinant catalytic subunit Calpha | Mus musculus |
Storage Stability | Organism |
---|---|
-70°C, purified recombinant catalytic subunit Cgamma, 0.5 M NaCl, 10 mM potassium phosphate, pH 6.9, 1 mM EDTA, 30% glycerol, on freeze-thaw cycle, stable up to one year | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + histone 3s | activity of the catalytic subunit Cgamma | Homo sapiens | ADP + phosphorylated histone 3s | - |
? | |
ATP + histone IIIS | activity of the catalytic subunit Cgamma | Mus musculus | ADP + phosphorylated histone IIIS | - |
? | |
ATP + Kemptide | activity of the catalytic subunit Calpha | Mus musculus | ADP + Kemptide phosphate | - |
? | |
ATP + Kemptide | PKA-derived substrate, activity of the catalytic subunit Cgamma | Homo sapiens | ADP + Kemptide phosphate | - |
? | |
ATP + RFARKGSLREKNV | protein kinase C-derived peptide, activity of the catalytic subunit Calpha | Mus musculus | ADP + RFARKG-phosphoserine-LREKNV | - |
? | |
ATP + RFARKGSLREKNV | protein kinase C-derived peptide, activity of the catalytic subunit Cgamma | Homo sapiens | ADP + RFARKG-phosphoserine-LREKNV | - |
? | |
ATP + RKRSRAE | cGPK-1-derived peptide, activity of the catalytic subunit Calpha | Mus musculus | ADP + RKR-phosphoserine-RAE | - |
? | |
ATP + RKRSRAE | cGPK-1-derived peptide, activity of the catalytic subunit Cgamma | Homo sapiens | ADP + RKR-phosphoserine-RAE | - |
? | |
ATP + RKRSRKE | cGPK-2-derived peptide, activity of the catalytic subunit Calpha | Mus musculus | ADP + RKR-phosphoserine-RKE | - |
? | |
ATP + RKRSRKE | cGPK-2-derived peptide, activity of the catalytic subunit Cgamma | Homo sapiens | ADP + RKR-phosphoserine-RKE | - |
? | |
ATP + RRLSSLRA | S6 kinase-derived peptide, activity of the catalytic subunit Calpha | Mus musculus | ADP + RRL-phosphoserine-phosphoserine-LRA | - |
? | |
ATP + RRLSSLRA | S6 kinase-derived peptide, activity of the catalytic subunit Cgamma | Homo sapiens | ADP + RRL-phosphoserine-phosphoserine-LRA | - |
? | |
additional information | substrate specificity, overview | Mus musculus | ? | - |
? | |
additional information | substrate specificity, overview | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 41000, isolated recombinant catalytic subunit Cgamma from Sf9 cells, SDS-PAGE | Homo sapiens |
monomer | 1 * 42000, isolated recombinant catalytic subunit Calpha from Escherichia coli, SDS-PAGE | Mus musculus |
More | determination of Stoke's radius of purified recombinant catalytic subunit Calpha | Mus musculus |
More | determination of Stoke's radius of purified recombinant catalytic subunit Cgamma | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Mus musculus |
PKA | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Mus musculus |
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Mus musculus |
7 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus | |
ATP | - |
Homo sapiens |