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Literature summary for 2.7.11.10 extracted from

  • Accardi, R.; Scalise, M.; Gheit, T.; Hussain, I.; Yue, J.; Carreira, C.; Collino, A.; Indiveri, C.; Gissmann, L.; Sylla, B.S.; Tommasino, M.
    IkappaB kinase beta promotes cell survival by antagonizing p53 functions through DELTANp73alpha phosphorylation and stabilization (2011), Mol. Cell. Biol., 31, 2210-2226.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged IKKalpha and IKKbeta in HEK-293 cells, coexpression of IKKbeta and GFP-tagged DELTANp73alpha protein in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information overexpressing a dominant-negative mutant of IKKbeta in 38E6E7HFK cells results in reduced levels of phospho-IkappaBalpha and a decrease in DELTANp73alpha protein levels, while no significant changes in DELTANp73alpha mRNA levels are observed Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
Bay 11-7082
-
 Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [IkappaB protein] Homo sapiens
-
 ADP + [IkappaB phosphoprotein]
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
38E6E7HFK cell
-
 Homo sapiens
-
HFK cell primary Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [DELTANp73alpha protein] phosphorylation at Ser422 by IKKbeta, IKKbeta is inactive with S422A mutant substrate. DELTANp73alpha i a dominant negative inhibitor of p53 and p73 Homo sapiens ADP + [DELTANp73alpha phosphoprotein]
-
 ?
ATP + [IkappaB protein]
-
 Homo sapiens ADP + [IkappaB phosphoprotein]
-
 ?

Synonyms

Synonyms Comment Organism
IkappaB kinase beta
-
 Homo sapiens
IKKbeta
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens

Expression

Organism Comment Expression
Homo sapiens IKKalpha and IKKbeta downregulation in 38E6E7HFK cells by siRNA expression down

General Information

General Information Comment Organism
metabolism IKKbeta is involved in the mechanism of regulation of the p73 and p53 network via DELTANp73alpha protein stabilization and inhibition of p53-regulated gene expression Homo sapiens
physiological function phosphorylation and degradation of IkappaBalpha is mediated by activated IKKalpha and IKKbeta in the canonical pathway of NF-kappaB. IkappaB kinase beta increases DELTANp73alpha protein stability independently of its ability to activate NF-kappaB. IKKbeta associates with and phosphorylates DELTANp73alpha protein at Ser422, leading to its accumulation in the nucleus, where it binds and represses several p53-regulated genes. IKKbeta and DELTANp73alpha protein cross talk in cancer-derived cell lines and primary cancers Homo sapiens