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Literature summary for 2.7.11.1 extracted from

  • Srinivasan, V.; Vaidyanathan, V.; Mondal, A.; Venkataramaiah, M.; Rajamohan, G.
    Functional characterization of a novel Mn2+ dependent protein serine/threonine kinase KpnK, produced by Klebsiella pneumoniae strain MGH78578 (2012), FEBS Lett., 586, 3778-3786.
    View publication on PubMed
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
gene kpnK, quantitative real-time RT-PCR expression analysis of wild-type and mutant enzymes, recombinant expression in Escherichia coli strain BL21(DE3) Klebsiella pneumoniae

Protein Variants

Protein Variants Comment Organism
additional information construction of the kpnK deletion mutant in strain MGH78578 Klebsiella pneumoniae

Localization

Localization Comment Organism GeneOntology No. Textmining
soluble the enzyme is a soluble protein with no transmembrane domains Klebsiella pneumoniae
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ low activity, can only partly substitute for Mn2+ Klebsiella pneumoniae
Mn2+ enzyme KpnK is dependent on Mn2+ Klebsiella pneumoniae
additional information no activity with Zn2+, Ni2+, and Mg2+ Klebsiella pneumoniae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Klebsiella pneumoniae KpnK specifically phosphorylates serine and threonine residues, and KpnK shows autophosphorylation activity ?
-
 ?
additional information Klebsiella pneumoniae MGH78578 KpnK specifically phosphorylates serine and threonine residues, and KpnK shows autophosphorylation activity ?
-
 ?

Organism

Organism UniProt Comment Textmining
Klebsiella pneumoniae
-
 gene kpnK or KPN_04169
-
Klebsiella pneumoniae MGH78578
-
 gene kpnK or KPN_04169
-

Purification (Commentary)

Purification (Comment) Organism
recombinant KpnK from Escherichia coli strain BL21(DE3) Klebsiella pneumoniae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + chicken basic protein
-
 Klebsiella pneumoniae ADP + phosphorylated chicken basic protein
-
 ?
ATP + chicken basic protein
-
 Klebsiella pneumoniae MGH78578 ADP + phosphorylated chicken basic protein
-
 ?
additional information KpnK specifically phosphorylates serine and threonine residues, and KpnK shows autophosphorylation activity Klebsiella pneumoniae ?
-
 ?
additional information KpnK specifically phosphorylates serine and threonine residues, and KpnK shows autophosphorylation activity Klebsiella pneumoniae MGH78578 ?
-
 ?

Synonyms

Synonyms Comment Organism
ESTK
-
 Klebsiella pneumoniae
eukaryotic-like serine/threonine kinase
-
 Klebsiella pneumoniae
KpnK
-
 Klebsiella pneumoniae
Ser/Thr kinase
-
 Klebsiella pneumoniae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
 Klebsiella pneumoniae

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 activity range Klebsiella pneumoniae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
 Klebsiella pneumoniae

pH Range

pH Minimum pH Maximum Comment Organism
7 7.5 narrow activity range Klebsiella pneumoniae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Klebsiella pneumoniae

Expression

Organism Comment Expression
Klebsiella pneumoniae gene knpK is induced by oxidative and nitrosative stress and other stress-associated challenge, overview up

General Information

General Information Comment Organism
malfunction the DkpnK mutant exhibits higher sensitivity to gastrointestinal and oxidative stresses. Deletion of kpnK result in a two to threefold increased susceptibility towards imipenem, cefepime, ceftriaxone and ceftazidime, gene kpnK participates in antibiotic resistance. Deletion of gene kpnK influences active efflux in Klebsiella pneumoniae. The downregulation of the MDR efflux pumps might be the molecular mechanism underlying the sensitivity of kpnK mutant to hydrophobic agents Klebsiella pneumoniae
physiological function involvement of ESTK in regulating bacterial physiology, stress response and drug resistance. Roles of gene kpnK in oxidative and nitrosative stress tolerance and in other stress-associated challenge, overview. Additional regulatory role of KpnK on MDR efflux pumps Klebsiella pneumoniae