Any feedback?
Literature summary for 2.7.11.1 extracted from
- The sole serine/threonine protein kinase and its cognate phosphatase from Aquifex aeolicus targets pyrimidine biosynthesis (2008), Mol. Cell. Biochem., 311, 199-213.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli as a His-tagged fusion protein | Aquifex aeolicus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.46 | - |
GTP | autophosphorylation | Aquifex aeolicus |  |
| 0.64 | - |
ATP | autophosphorylation | Aquifex aeolicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
SDS-PAGE | Aquifex aeolicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aquifex aeolicus | O66844 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | enzyme is autophosphorylated on a threonine residue | Aquifex aeolicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using Ni2+-affinity chromatography | Aquifex aeolicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + AaSTPK | enzyme is autophosphorylated on a threonine residue | Aquifex aeolicus | ADP + phosphorylated-AaSTPK | - |
? | |
| ATP + CPS.A subunit | AaSTPK also phosphorylates one threonine residue on the carbamoyl phosphate synthetase, CPS.A subunit | Aquifex aeolicus | ADP + phosphorylated-CPS.A subunit | - |
? | |
| GTP + AaSTPK | enzyme is autophosphorylated on a threonine residue | Aquifex aeolicus | GDP + phosphorylated-AaSTPK | - |
? | |
| GTP + CPS.A subunit | - |
Aquifex aeolicus | GDP + phosphorylated-CPS.A subunit | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | gel filtration | Aquifex aeolicus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AaSTPK | - |
Aquifex aeolicus |
| serine-threonine kinase | - |
Aquifex aeolicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
assay at | Aquifex aeolicus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00006 | - |
GTP | autophosphorylation | Aquifex aeolicus | |
| 0.00016 | - |
ATP | autophosphorylation | Aquifex aeolicus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
assay at | Aquifex aeolicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| MgATP2- | preferred substrate | Aquifex aeolicus | |
| MnATP2- | - |
Aquifex aeolicus | |
| MnGTP2- | - |
Aquifex aeolicus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
