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Literature summary for 2.7.11.1 extracted from
- Open reading frame sso2387 from the archaeon Sulfolobus solfataricus encodes a polypeptide with protein-serine kinase activity (2003), J. Bacteriol., 185, 3436-3445.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| orf sso2387, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) in inclusion bodies | Saccharolobus solfataricus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Saccharolobus solfataricus |  |
| tamoxifen | - |
Saccharolobus solfataricus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can only poorly substitute for Mn2+ | Saccharolobus solfataricus | |
| Mg2+ | less effective than Mn2+ | Saccharolobus solfataricus | |
| Mn2+ | preferred metal ion cofactor, especially for the autophosphorylation reaction | Saccharolobus solfataricus | |
| Ni2+ | can only poorly substitute for Mn2+ | Saccharolobus solfataricus | |
| Zn2+ | can only partially substitute for Mn2+ | Saccharolobus solfataricus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | Saccharolobus solfataricus | - |
ADP + a phosphoprotein | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
orf sso2387 | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme performs autophosphorylation at Ser548, determination of sites of autophosphorylation by mass spectrometry in two areas, one immediately N terminal to the region corresponding to subdomain I of eukaryotic protein kinases, and the second N terminal to the presumed activation loop located between subdomains VII and VIII, autophosphorylation of rSsoPK2 can be uncoupled from the phosphorylation of exogenous proteins by manipulation of the temperature or mutagenic alteration of the enzyme, autophosphorylation is detected only at temperatures above 60°C, whereas phosphorylation of exogenous proteins is detectable at 37°C | Saccharolobus solfataricus |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| solubilization from inclusion bodies after recombinant expression in Escherichia coli by treatment with 5 M urea | Saccharolobus solfataricus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
substrate specificity of the recombinant enzyme, overview | Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + a protein | - |
Saccharolobus solfataricus | ADP + a phosphoprotein | - |
? | |
| ATP + a protein | PK2 phosphorylate itself as well as several exogenous proteins, including mixed histones, casein, bovine serum albumin, and reduced carboxyamidomethylated and maleylated lysozyme, on serine residues, overview, autophosphorylation of rSsoPK2 can be uncoupled from the phosphorylation of exogenous proteins by manipulation of the temperature or mutagenic alteration of the enzyme, autophosphorylation is detected only at temperatures above 60°C, whereas phosphorylation of exogenous proteins is detectable at 37°C | Saccharolobus solfataricus | ADP + a phosphoprotein | - |
? | |
| ATP + bovine serum albumin | - |
Saccharolobus solfataricus | ADP + phosphorylated bovine serum albumin | - |
? | |
| ATP + casein | - |
Saccharolobus solfataricus | ADP + phosphocasein | - |
? | |
| ATP + histone | - |
Saccharolobus solfataricus | ADP + phosphorylated histone | - |
? | |
| ATP + lysozyme | reduced carboxyamidomethylated and maleylated lysozyme | Saccharolobus solfataricus | ADP + phopshorylated lysozyme | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SsoPK2 | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
phosophorylation of exogenous proteins | Saccharolobus solfataricus |
| 60 | - |
above, autophosphorylation activity | Saccharolobus solfataricus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Saccharolobus solfataricus | |
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