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Literature summary for 2.7.11.1 extracted from

  • Min, X.; Lee, B.H.; Cobb, M.H.; Goldsmith, E.J.
    Crystal structure of the kinase domain of WNK1, a kinase that causes a hereditary form of hypertension (2004), Structure, 12, 1303-1311.
    View publication on PubMed
Search for more literature for 2.7.11.1

Activating Compound

Activating Compound Comment Organism Structure
additional information activation loop structure and conformation, WNK1 performs autophosphorylation at S382 for activation Rattus norvegicus

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged low activity WNK1 kinase domain mutant S382A, comprising residues 194-483, in Escherichia coli Rattus norvegicus

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant low activity WNK1 mutant S382A, wild-type and selenomethionine-labeled, hanging drop vapour diffusion method, 16°C, mixing of 0.002 ml of protein solution and of well solution, the latter containing 24% PEG monomethyl ester 2000, 0.3 M NaCl, 0.1 M HEPES, pH 7.0-8.0, 3 days, stabilization with 15% glycerol, X-ray diffraction structure determination and analysis at 1.8 A resolution Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E268Q site-directed mutagenesis, residue is probably important in enzyme stabilization since the mutant enzyme cannot be expressed recombinantly Rattus norvegicus
S382A site-directed mutagenesis, mutant remains in the low activity form Rattus norvegicus
V318E/A448K site-directed mutagenesis, the mutant shows 50-60% reduced activity compared to the wild-type WNK1 Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus WNK1 causes a hereditary form of hypertension, pseudohypoaldosteronism type II ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein WNK1 performs autophosphorylation at S382 for activation Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged low activity WNK1 kinase domain mutant S382A, comprising residues 194-483, wild-type and selenomethionine-labeled, from Escherichia coli by chelating affinity and ion exchange chromatography, and gel filtration Rattus norvegicus

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate active site and activation loop structure and conformation, Lys233, Cys250, Asp368, and Thr386 are important for activity, the substrate binding groove determines the substrate specificity via residues Val318 and Ala448 Rattus norvegicus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + synaptotagmin 2 WNK1 specific substrate Rattus norvegicus ADP + phosphorylated synaptotagmin 2
-
 ?
additional information WNK1 causes a hereditary form of hypertension, pseudohypoaldosteronism type II Rattus norvegicus ?
-
 ?
additional information WNK1 performs autophosphorylation at S382 for activation Rattus norvegicus ?
-
 ?

Subunits

Subunits Comment Organism
More kinase domain structure of WNK1 Rattus norvegicus

Synonyms

Synonyms Comment Organism
WNK1
-
 Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
 assay at Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
ATP binding site structure involving Lys233 Rattus norvegicus