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Literature summary for 2.7.11.1 extracted from
- Cloning, overexpression, and characterization of a serine/threonine protein kinase pknI from Mycobacterium tuberculosis H37Rv (2004), Protein Expr. Purif., 36, 82-89.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene pknI, DNA and amino acid sequence determination and analysis, NaCl-inducible expression of N-terminally His-tagged full-length and cytoplasmic domain of PknI in Escherichia coli strain BL21 in inclusion bodies | Mycobacterium tuberculosis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | enzyme contains a cytoplasmic domain | Mycobacterium tuberculosis | 5737 | - |
| membrane | - |
Mycobacterium tuberculosis | 16020 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | can partly substitute for Mn2+, activates | Mycobacterium tuberculosis |  |
| Mg2+ | activates | Mycobacterium tuberculosis | |
| Mn2+ | strictly required, activates | Mycobacterium tuberculosis | |
| additional information | no activity with Mg2+ alone, no activity with Cu2+ or Zn2+ | Mycobacterium tuberculosis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE | Mycobacterium tuberculosis |
| 61805 | - |
x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE | Mycobacterium tuberculosis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged full-length and cytoplasmic domain of PknI from Escherichia coli strain BL21 by nickel affinity chromatography | Mycobacterium tuberculosis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| refolding of recombinant enzyme | Mycobacterium tuberculosis |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61805, amino acid sequence calculation, x * 55000, recombinant cytoplasmic domain, SDS-PAGE, x * 80000-85000, recombinant full-length PknI, migrates probably as a doublet, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PknI | - |
Mycobacterium tuberculosis |
| serine/threonine protein kinase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mycobacterium tuberculosis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mycobacterium tuberculosis | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mycobacterium tuberculosis | amino acid sequence calculation | - |
5.5 |
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