Activating Compound | Comment | Organism | Structure |
---|---|---|---|
putrescine | aliphatic, positively charged polyamine, required for in vitro activity of ORF47, polyamine depletion leads to 80% reduced activity | Human alphaherpesvirus 3 | |
spermidine | aliphatic, positively charged polyamine, required for in vitro activity of ORF47, polyamine depletion leads to 80% reduced activity | Human alphaherpesvirus 3 | |
spermine | aliphatic, positively charged polyamine, required for in vitro activity of ORF47, polyamine depletion leads to 80% reduced activity | Human alphaherpesvirus 3 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | strongly inhibits casein kinase II | Homo sapiens | |
additional information | ORF47 kinase is resistant to heparin, host cell pretreatment prior to virus infection with D,L-alpha-difluoromethylornithine reduces intracellular polyamine concentration and thus inhibits the viral enzyme ORF47 | Human alphaherpesvirus 3 |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Human alphaherpesvirus 3 | - |
VZV-32, propagated in MeWo human melanoma cells | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HeLa cell | - |
Homo sapiens | - |
melanoma cell | - |
Homo sapiens | - |
MeWo cell | melanoma cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + casein | - |
Human alphaherpesvirus 3 | ADP + phosphorylated casein | - |
? | |
ATP + IgG2A heavy chain | substrate protein from mouse | Human alphaherpesvirus 3 | ADP + phosphorylated IgG2A heavy chain | - |
? | |
ATP + maltose-binding protein | - |
Human alphaherpesvirus 3 | ADP + phosphorylated maltose-binding protein | - |
? | |
ATP + ORF62 protein | protein substrate from Varicella-Zoster virus, no activity with a truncated ORF62 mutant | Human alphaherpesvirus 3 | ADP + ORF62 phosphoprotein | - |
? | |
ATP + ORF63 protein | protein substrate from Varicella-Zoster virus | Human alphaherpesvirus 3 | ADP + ORF63 phosphoprotein | - |
? | |
ATP + Varicella-Zoster viral gB protein | phosphorylation at S34 in the sequence QSEDIT | Homo sapiens | ADP + phosphorylated Varicella-Zoster viral gB protein | - |
? | |
additional information | CKII phosphorylates serine or threonine residues with an acidic amino acid in the +2 or +3 position | Homo sapiens | ? | - |
? | |
additional information | the minimal consensus sequence of ORF47 is S/T-X-D/E-D/E, no activity of ORF47 with glutathione S-transferase, or Varicella-Zoster viral gB protein | Human alphaherpesvirus 3 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
casein kinase II | - |
Homo sapiens |
CKII | - |
Homo sapiens |
ORF47 protein | - |
Human alphaherpesvirus 3 |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Human alphaherpesvirus 3 |