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Literature summary for 2.7.11.1 extracted from

  • Tamaskovic, R.; Bichsel, S.J.; Rogniaux, H.; Stegert, M.R.; Hemmings, B.A.
    Mechanism of Ca2+-mediated regulation of NDR protein kinase through autophosphorylation and phosphorylation by an upstream kinase (2003), J. Biol. Chem., 278, 6710-6718.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information NDR1 is activated by phosphorylation at S281, T444, and T74 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expresion of wild-type and mutant GST-fusion NDR1 in Escherichia coli strain BL21(DE3), expression of HA-tagged NDR1 in COS-1 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
S281A site-directed mutagenesis, mutant shows abolished autophosphorylation at T444 Homo sapiens
T444A site-directed mutagenesis, mutant shows almost unaltered autophosphorylation at S281 compared to the wild-type enzyme Homo sapiens
T74A site-directed mutagenesis, mutant shows only slightly reduced kinase activity and autophosphorylation compared to the wild-type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ has regulatory function for NDR protein kinase, mechanism, overview Homo sapiens
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Homo sapiens mechanism of Ca2+-mediated regulation of NDR protein kinase through autophosphorylation and phosphorylation by an upstream kinase ?
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein mapping of autophosphorylation sites, NDR1 is activated by phosphorylation at S281, T444, and T74 dependent on intracellular Ca2+ Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and mutant GST-fusion NDR1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + KKRNRRLSVA peptide substrate of NDR1 Homo sapiens ADP + KKRNRRL-phosphoserine-VA
-
?
additional information mechanism of Ca2+-mediated regulation of NDR protein kinase through autophosphorylation and phosphorylation by an upstream kinase Homo sapiens ?
-
?
additional information NDR1 performs autophosphorylation at S281, T444, and T74 dependent on intracellular Ca2+ Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
NDR protein kinase
-
Homo sapiens
NDR1
-
Homo sapiens
nuclear Dbf2-related protein kinase
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens