Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | NDR1 is activated by phosphorylation at S281, T444, and T74 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expresion of wild-type and mutant GST-fusion NDR1 in Escherichia coli strain BL21(DE3), expression of HA-tagged NDR1 in COS-1 cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
S281A | site-directed mutagenesis, mutant shows abolished autophosphorylation at T444 | Homo sapiens |
T444A | site-directed mutagenesis, mutant shows almost unaltered autophosphorylation at S281 compared to the wild-type enzyme | Homo sapiens |
T74A | site-directed mutagenesis, mutant shows only slightly reduced kinase activity and autophosphorylation compared to the wild-type enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | has regulatory function for NDR protein kinase, mechanism, overview | Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | mechanism of Ca2+-mediated regulation of NDR protein kinase through autophosphorylation and phosphorylation by an upstream kinase | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | mapping of autophosphorylation sites, NDR1 is activated by phosphorylation at S281, T444, and T74 dependent on intracellular Ca2+ | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant GST-fusion NDR1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + KKRNRRLSVA | peptide substrate of NDR1 | Homo sapiens | ADP + KKRNRRL-phosphoserine-VA | - |
? | |
additional information | mechanism of Ca2+-mediated regulation of NDR protein kinase through autophosphorylation and phosphorylation by an upstream kinase | Homo sapiens | ? | - |
? | |
additional information | NDR1 performs autophosphorylation at S281, T444, and T74 dependent on intracellular Ca2+ | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NDR protein kinase | - |
Homo sapiens |
NDR1 | - |
Homo sapiens |
nuclear Dbf2-related protein kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |