Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activation loop structure | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of GSK3beta in Escherichia coli in fusion with a Leu-Glu-His6-tag at the C-terminus | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant Leu-Glu-His6-tagged GSK3beta in complex with ATP or ATP analogue AMP-PNP, 10 mg/ml protein, 2 mM ATP or AMP-PNP, 12-14% w/v PEG 6000, 100 mM NaCl, 5 mM MgCl2, 10% v/v glycerol, in 100 mM HEPES-NaOH, pH 7.5, hanging drop vapor diffusion method, 4°C, several days, soaking in 0.1 mM ethylmercuric thiosalyclate at pH 7.5 for 1 h, cryoprotection by 30% w/v D-sorbitol, X-ray diffraction structure determination and analysis at 1.7-2.6 A resolution | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP-PNP | ATP analogue adenosine 5'-(beta,gamma-imino)triphosphate | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | binding site structure | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme participates in Alzheimer's disease | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant Leu-Glu-His6-tagged GSK3beta from Escherichia coli | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [tau-protein] | substrate of GSK3beta in brain | Homo sapiens | ADP + O-phospho-[tau-protein] | - |
? | |
additional information | the enzyme participates in Alzheimer's disease | Homo sapiens | ? | - |
? | |
additional information | substrate specificity of GSK3beta | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycogen synthase kinase 3beta | - |
Homo sapiens |
GSK3beta | - |
Homo sapiens |
More | see also EC 2.7.11.26 | Homo sapiens |
tau-protein kinase I | - |
Homo sapiens |
TPK I | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | binding site structure | Homo sapiens |