General Stability | Organism |
---|---|
diluted solutions are unstable | Bos taurus |
repeated freeze-thawing inactivates | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Bos taurus | |
additional information | heparin, Ca2+/calmodulin, phosphatidylserine and/or diolein (in the absence or presence of Ca2+); protein inhibitor of cAMP-dependent protein kinase (protamine as substrate), monoclonal antibodies to catalytic domain of protein kinase C from rat brain | Bos taurus | |
NaCl | bovine serum albumin, not protamine as substrate | Bos taurus | |
O-phosphoprotamine | bovine serum albumin, not protamine as substrate | Bos taurus | |
spermine | bovine serum albumin, not protamine as substrate | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Bos taurus | 5739 | - |
soluble | - |
Bos taurus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 1.5 mM (mitochondrial kinase) | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
mitochondrial kinase | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
5.11 | - |
- |
Bos taurus |
Storage Stability | Organism |
---|---|
-20°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, at least 6 months | Bos taurus |
0-4°C, in 0.05 M imidazole chloride, pH 7.3, 10% glycerol, 1 mM DTT, protease inhibitors, 1 mM EDTA, up to 3 weeks | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + bovine serum albumin | phosphorylated at 40-70% the rate of protamine | Bos taurus | ? | - |
? | |
ATP + glycogen synthase | phosphorylated at 15% the rate of protamine | Bos taurus | ? | - |
? | |
ATP + histone H1 | phosphorylated at 80% the rate of protamine | Bos taurus | ? | - |
? | |
ATP + protamine | preferred substrate | Bos taurus | ADP + O-phosphoprotamine | - |
? | |
ATP + protamine | phosphorylates little, if any activity with branched-chain alpha-keto acid dehydrogenase, pyruvate dehydrogenase, casein, ovalbumin or histone H2B | Bos taurus | ADP + O-phosphoprotamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Bos taurus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.1 | 8.2 | protamine as substrate | Bos taurus |
6.7 | - |
bovine serum albumin as substrate | Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.8 | 8.8 | about 85% of maximal activity at pH 5.8 and about 80% of maximal activity at pH 8.8, protamine as substrate | Bos taurus |
6.6 | 6.8 | about half-maximal activity at pH 6.6 and pH 6.8, bovine serum albumin as substrate | Bos taurus |