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Literature summary for 2.7.1.B26 extracted from

  • Shao, L.; Chen, J.; Wang, C.; Li, J.A.; Tang, Y.; Chen, D.; Liu, W.
    Characterization of a key aminoglycoside phosphotransferase in gentamicin biosynthesis (2013), Bioorg. Med. Chem. Lett., 23, 1438-1441.
    View publication on PubMed
Search for more literature for 2.7.1.B26

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Micromonospora echinospora

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 kanamycin B pH 7.5, 37°C Micromonospora echinospora
0.014
-
 neomycin B pH 7.5, 37°C Micromonospora echinospora
0.018
-
 kanamycin A pH 7.5, 37°C Micromonospora echinospora
0.048
-
 amikacin pH 7.5, 37°C Micromonospora echinospora

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
 x * 30000, SDS-PAGE and calculated Micromonospora echinospora

Organism

Organism UniProt Comment Textmining
Micromonospora echinospora Q6QVT6
-
-
Micromonospora echinospora ATCC 15835 Q6QVT6
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein Micromonospora echinospora

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GTP + amikacin
-
 Micromonospora echinospora GDP + amikacin 3'-phosphate
-
 ?
GTP + amikacin
-
 Micromonospora echinospora ATCC 15835 GDP + amikacin 3'-phosphate
-
 ?
GTP + gentamicin JI-20A
-
 Micromonospora echinospora GDP + gentamicin JI-20A 3'-phosphate modification occurs solely at the 3'-OH ?
GTP + gentamicin JI-20A
-
 Micromonospora echinospora ATCC 15835 GDP + gentamicin JI-20A 3'-phosphate modification occurs solely at the 3'-OH ?
GTP + kanamycin A
-
 Micromonospora echinospora GDP + kanamycin A 3'-phosphate
-
 ?
GTP + kanamycin A
-
 Micromonospora echinospora ATCC 15835 GDP + kanamycin A 3'-phosphate
-
 ?
GTP + kanamycin B
-
 Micromonospora echinospora GDP + kanamycin B 3'-phosphate
-
 ?
GTP + kanamycin B
-
 Micromonospora echinospora ATCC 15835 GDP + kanamycin B 3'-phosphate
-
 ?
GTP + neomycin B
-
 Micromonospora echinospora GDP + neomycin B 3'-phosphate
-
 ?
GTP + neomycin B
-
 Micromonospora echinospora ATCC 15835 GDP + neomycin B 3'-phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE and calculated Micromonospora echinospora

Synonyms

Synonyms Comment Organism
APH(3')
-
 Micromonospora echinospora
biosynthetic aminoglycoside 3'-phosphotransferase
-
 Micromonospora echinospora
GnTI
-
 Micromonospora echinospora

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.07
-
 kanamycin B pH 7.5, 37°C Micromonospora echinospora
0.12
-
 amikacin pH 7.5, 37°C Micromonospora echinospora
0.122
-
 neomycin B pH 7.5, 37°C Micromonospora echinospora
0.125
-
 kanamycin A pH 7.5, 37°C Micromonospora echinospora

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.5
-
 amikacin pH 7.5, 37°C Micromonospora echinospora
5.8
-
 kanamycin B pH 7.5, 37°C Micromonospora echinospora
6.7
-
 kanamycin A pH 7.5, 37°C Micromonospora echinospora
8.6
-
 neomycin B pH 7.5, 37°C Micromonospora echinospora