Literature summary for 2.7.1.95 extracted from

Shakya, T.; Wright, G.D.
Nucleotide selectivity of antibiotic kinases (2010), Antimicrob. Agents Chemother., 54, 1909-1913.

Search for more literature for 2.7.1.95

Application

Application	Comment	Organism
analysis	competitive assay that mimics in vivo nucleotide triphosphate concentrations and usage by the enzyme. Downstream analysis of reaction products by high-performance liquid chromatography enables the determination of partitioning of phosphate flux from nucleotide triphosphate donors to antibiotics	Enterococcus casseliflavus

Inhibitors

Inhibitors	Comment	Organism	Structure
Adenylyl imidodiphosphate	-	Enterococcus casseliflavus
additional information	not inhibitory: guanylyl imidodiphosphate	Enterococcus casseliflavus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0106	-	ATP	pH 7.5, 37°C	Enterococcus casseliflavus

Organism

Organism	UniProt	Comment	Textmining
Enterococcus casseliflavus	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + neomycin	-	Enterococcus casseliflavus	ADP + neomycin 3'-phosphate	-	?
additional information	under physiologic concentrationsof NTPs, enzyme exclusively uses ATP	Enterococcus casseliflavus	?	-	?

Synonyms

Synonyms	Comment	Organism
aph(3'')-IIIa	-	Enterococcus casseliflavus

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1.59	-	ATP	pH 7.5, 37°C	Enterococcus casseliflavus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0142	-	Adenylyl imidodiphosphate	pH 7.5, 37°C	Enterococcus casseliflavus

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
150	-	ATP	pH 7.5, 37°C	Enterococcus casseliflavus

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Release 2023.1 (January 2023)