Cloned (Comment) | Organism |
---|---|
overexpression of aminoglycoside phosphotransferase-IIIa, i.e. APH3'-IIIa in Escherichia coli | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
kanamycin A | pH 6.5, 37°C, isoenzyme APH3'-IIIa | Escherichia coli | |
0.013 | - |
kanamycin A | pH 7.5, 37°C, isoenzyme APH3'-IIIa | Escherichia coli | |
0.022 | - |
ADP | pH 7.5, 37°C, isoenzyme APH3'-IIIa | Escherichia coli | |
0.028 | - |
ATP | pH 7.5, 37°C, isoenzyme APH3'-IIIa | Escherichia coli | |
1.32 | - |
3'-phosphokanamycin | pH 7.5, 37°C, isoenzyme APH3'-IIIa | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + kanamycin = ADP + kanamycin 3'-phosphate | isoenzyme APH3'-IIIa follows a Theorell-Chance mechanism | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + kanamycin | isoenzyme APH3'-IIIa | Escherichia coli | ADP + kanamycin 3'-phosphate | - |
r |
Synonyms | Comment | Organism |
---|---|---|
aminoglycoside phosphotransferase 3'-IIIa, i.e. APH3'-IIIa | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
ATP | pH 7.5, 37°C, isoenzyme APH3'-IIIa | Escherichia coli | |
1.76 | - |
ATP | pH 7.5, 37°C, isoenzyme APH3'-IIIa | Escherichia coli | |
1.79 | - |
kanamycin A | pH 7.5, 37°C, isoenzyme APH3'-IIIa | Escherichia coli |