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Literature summary for 2.7.1.94 extracted from
- Distinct 1-monoacylglycerol and 2-monoacylglycerol kinase activities of diacylglycerol kinase isozymes (2016), Biochim. Biophys. Acta, 1864, 1170-1176.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| recombinant expression of His6-tagged enzyme AGK from vector pSF-CMV-NH2-His-EKT3 in COS-7 cells | Mus musculus |
| recombinant expression of human His6-tagged DGK isozyme theta from vector pSF-CMV-NH2-His-EKT3 in COS-7 cells | Homo sapiens |
| recombinant expression of human p3×FLAG-CMV-DGK isozyme delta2 in COS-7 cells | Homo sapiens |
| recombinant expression of human p3×FLAG-CMV-DGK isozyme epsilon in COS-7 cells | Homo sapiens |
| recombinant expression of human p3×FLAG-CMV-DGK isozyme eta1 in COS-7 cells | Homo sapiens |
| recombinant expression of human p3×FLAG-CMV-DGK isozyme gamma in COS-7 cells | Homo sapiens |
| recombinant expression of human p3×FLAG-CMV-DGK isozyme kappa in COS-7 cells | Homo sapiens |
| recombinant expression of human p3×FLAG-CMV-DGK isozyme zeta1 in COS-7 cells | Homo sapiens |
| recombinant expression of pig p3×FLAG-CMV-DGK isozyme alpha in COS-7 cells | Sus scrofa |
| recombinant expression of rat p3×FLAG-CMV-DGK isozyme beta in COS-7 cells | Rattus norvegicus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Homo sapiens |  |
| Mg2+ | required | Rattus norvegicus | |
| Mg2+ | required | Sus scrofa | |
| Mg2+ | required | Mus musculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acylglycerol | Mus musculus | - |
ADP + acyl-sn-glycerol 3-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme gamma | - |
| Homo sapiens | - |
isozyme theta | - |
| Homo sapiens | - |
isozyme delta2 | - |
| Homo sapiens | - |
isozyme epsilon | - |
| Homo sapiens | - |
isozyme eta1 | - |
| Homo sapiens | - |
isozyme kappa | - |
| Homo sapiens | - |
isozyme zeta1 | - |
| Mus musculus | Q9ESW4 | - |
- |
| Rattus norvegicus | - |
isozyme beta | - |
| Sus scrofa | - |
isozyme alpha | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant human His6-tagged DGK isozyme theta from COS-7 cells by nickel affinity chromatography | Homo sapiens |
| recombinant murine His6-tagged enzyme AGK from COS-7 cells by nickel affinity chromatography | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + acylglycerol | - |
Mus musculus | ADP + acyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + acylglycerol | the enzyme acts on both 1- and 2-acylglycerols, substrate specificity, overview | Homo sapiens | ADP + acyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + acylglycerol | the enzyme acts on both 1- and 2-acylglycerols, substrate specificity, overview | Rattus norvegicus | ADP + acyl-sn-glycerol 3-phosphate | - |
? | |
| ATP + acylglycerol | the enzyme acts on both 1- and 2-acylglycerols, substrate specificity, overview | Sus scrofa | ADP + acyl-sn-glycerol 3-phosphate | - |
? | |
| additional information | in addition to its main activity, phosphorylation of 1,2-diacyl-sn-glycerol to 1,2-diacyl-sn-glycerol 3-phosphate, EC 2.7.1.107, the enzyme also shows acylglycerol kinase activity, EC 2.7.1.94. Both monoacylglycerol kinase activities are low | Homo sapiens | ? | - |
? | |
| additional information | in addition to its main activity, phosphorylation of 1,2-diacyl-sn-glycerol to 1,2-diacyl-sn-glycerol 3-phosphate, EC 2.7.1.107, the enzyme also shows acylglycerol kinase activity, EC 2.7.1.94. Both monoacylglycerol kinase activities are very low | Homo sapiens | ? | - |
? | |
| additional information | in addition to its main activity, phosphorylation of 1,2-diacyl-sn-glycerol to 1,2-diacyl-sn-glycerol 3-phosphate, EC 2.7.1.107, the enzyme also shows acylglycerol kinase activity, EC 2.7.1.94. The 2-MGK activity is very low | Homo sapiens | ? | - |
? | |
| additional information | in addition to its main activity, phosphorylation of 1,2-diacyl-sn-glycerol to 1,2-diacyl-sn-glycerol 3-phosphate, EC 2.7.1.107, the enzyme also shows acylglycerol kinase activity, EC 2.7.1.94. The 2-MGK activity is very low | Rattus norvegicus | ? | - |
? | |
| additional information | in addition to its main activity, phosphorylation of 1,2-diacyl-sn-glycerol to 1,2-diacyl-sn-glycerol 3-phosphate, EC 2.7.1.107, the enzyme also shows acylglycerol kinase activity, EC 2.7.1.94. The 2-MGK activity is very low | Sus scrofa | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 1-MGK | - |
Homo sapiens |
| 1-MGK | - |
Rattus norvegicus |
| 1-MGK | - |
Sus scrofa |
| 1-monoacylglycerol kinase | - |
Homo sapiens |
| 1-monoacylglycerol kinase | - |
Rattus norvegicus |
| 1-monoacylglycerol kinase | - |
Sus scrofa |
| 2-MGK | - |
Homo sapiens |
| 2-MGK | - |
Rattus norvegicus |
| 2-MGK | - |
Sus scrofa |
| 2-monoacylglycerol kinase | - |
Homo sapiens |
| 2-monoacylglycerol kinase | - |
Rattus norvegicus |
| 2-monoacylglycerol kinase | - |
Sus scrofa |
| acylglycerol kinase | - |
Mus musculus |
| AGK | - |
Mus musculus |
| More | cf. EC 2.7.1.107 | Homo sapiens |
| More | cf. EC 2.7.1.107 | Rattus norvegicus |
| More | cf. EC 2.7.1.107 | Sus scrofa |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Rattus norvegicus |
| 30 | - |
assay at | Sus scrofa |
| 30 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Homo sapiens |
| 7.2 | - |
assay at | Rattus norvegicus |
| 7.2 | - |
assay at | Sus scrofa |
| 7.2 | - |
assay at | Mus musculus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
| ATP | - |
Rattus norvegicus | |
| ATP | - |
Sus scrofa | |
| ATP | - |
Mus musculus | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (lambda and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date | Homo sapiens |
| evolution | comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date | Homo sapiens |
| evolution | comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date | Rattus norvegicus |
| evolution | comprehensive analysis on the 1-monoacylglycerol kinase (1-MGK) and 2-monoacylglycerol kinase (2-MGK) activities of ten diacylglyceol kinase (DGK) isozymes, EC 2.7.1.107, from different organisms. Type I (alpha, beta, and gamma), type II (delta, eta, and kappa) and type III (epsilon) DGKs have 7.9-19.2% 2-MGK activity compared to their DGK activities, whereas their 1-MGK activities are below 3.0%. Both the 1-MGK and 2-MGK activities of the type IV DGKs (zeta and iota) are below 1% relative to their DGK activities. Type V DGKtheta has approximately 6% 1-MGK activity and below 2% 2-MGK activity compared to its DGK activity. Purified DGKtheta exhibits the same results, indicating that its 1-MGK activity is intrinsic. DGK isozymes are categorized into three types with respect to their 1-MGK and 2-MGK activities: those having (1) 2-MGK activity relatively stronger than their 1-MGK activity (types I-III), (2) only negligible 1-MGK and 2-MGK activities (type IV), and (3) 1-MGK activity stronger than its 2-MGK activity (type V). The 1-MGK activity of DGKtheta and the 2-MGK activity of DGKalpha are stronger than those of the acylglycerol kinase reported as 1-MGK and 2-MGK to date | Sus scrofa |
| malfunction | presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme | Homo sapiens |
| malfunction | presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme | Rattus norvegicus |
| malfunction | presence or absence of 1-MGK and 2-MGK activities may be essential to the pathophysiological functions of each DGK isozyme | Sus scrofa |
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