Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | not inhibited by rac-1-hexadecanoylglycerol, rac-1,2-dihexadecanoylglycerol, rac-1-hexadecyl-2-hexadecanoylglycerol or 2-hexadecylglycerol | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.6 | - |
ATP | - |
Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | absolute requirement for a divalent metal ion, Mg2+ is most effective | Oryctolagus cuniculus | |
Mn2+ | about 50% as effective as Mg2+ in activation | Oryctolagus cuniculus | |
additional information | little activity with Zn2+, Ba2+, Cu2+, Ca2+, Hg2+, Sn2+, Sr2+, Ni2+, Fe2+, NaCl and KCl | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-alkyl-sn-glycerol | Oryctolagus cuniculus | involved in the biosynthesis of complex ether-linked glycerolipids | ADP + 1-alkyl-sn-glycero-3-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
adult male New Zealand white rabbit | - |
Purification (Comment) | Organism |
---|---|
partial | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
harderian gland | pink portions of | Oryctolagus cuniculus | - |
Storage Stability | Organism |
---|---|
-20°C, whole microsomal preparation, 0.25 M sucrose, 5 mM dithiothreitol, at least 2 months, stable | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 1-alkyl-sn-glycerol | stereoselective transfer of the terminal phosphate of ATP to position 3 of the substrate, ATP is an absolute requirement for enzymatic activity | Oryctolagus cuniculus | ADP + 1-alkyl-sn-glycero-3-phosphate | - |
? | |
ATP + 1-alkyl-sn-glycerol | involved in the biosynthesis of complex ether-linked glycerolipids | Oryctolagus cuniculus | ADP + 1-alkyl-sn-glycero-3-phosphate | - |
? | |
ATP + 1-O-hexadecyl-sn-glycerol | - |
Oryctolagus cuniculus | ADP + 1-O-hexadecyl-sn-glycero-3-phosphate | - |
? | |
ATP + 1-octadecyl-sn-glycerol | - |
Oryctolagus cuniculus | ADP + 1-octadecyl-sn-glycero-3-phosphate | - |
? | |
additional information | not: alkylethylene glycols, only trace activity with 1-thio-hexadecyl-2,3-propanediol | Oryctolagus cuniculus | ? | - |
? | |
additional information | not: S-alkyl-alkylglycerols, 2-hexadecylglycerol, 3-hexadecyl-sn-glycerol, 1-hexadecylethylene glycol | Oryctolagus cuniculus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.1 | - |
0.1 M Tris-maleate buffer | Oryctolagus cuniculus |
7.1 | - |
no sharp pH optimum | Oryctolagus cuniculus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.1 | 8.1 | about 66% of maximal activity at pH 6.1 and 8.1 | Oryctolagus cuniculus |