Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | enzyme is induced by growth on myo-inositol | Klebsiella aerogenes |
General Stability | Organism |
---|---|
purified enzyme is unstable to repeated freezing and thawing in 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol | Klebsiella aerogenes |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.097 | - |
2-deoxy-5-keto-D-gluconic acid | - |
Klebsiella aerogenes | |
0.22 | - |
ATP | - |
Klebsiella aerogenes |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | MgCl2 is included in assay mixture on the assumption that it is required | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
225000 | - |
sucrose density gradient centrifugation | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-5-keto-D-gluconic acid | Klebsiella aerogenes | involved in myo-inositol degradation | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
ATP + 2-deoxy-5-keto-D-gluconic acid | Klebsiella aerogenes constitutive | involved in myo-inositol degradation | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
C1, constitutive strain | - |
Klebsiella aerogenes constitutive | - |
C1, constitutive strain | - |
Purification (Comment) | Organism |
---|---|
partial, 20fold, not seperated from 2-deoxy-5-keto-D-gluconic acid 6-phosphate aldolase | Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
13.6 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
0°C, 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol, 1 month or more, stable | Klebsiella aerogenes |
4°C, enzyme of DEAE-Sephadex fractions, 20 mM potassium phosphate buffer, pH 7, 5 mM EDTA, 10 mM 2-mercaptoethanol, 0.2 M KCl, 5 months, stable | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 2-deoxy-5-keto-D-gluconic acid | - |
Klebsiella aerogenes | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
ATP + 2-deoxy-5-keto-D-gluconic acid | involved in myo-inositol degradation | Klebsiella aerogenes | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
ATP + 2-deoxy-5-keto-D-gluconic acid | - |
Klebsiella aerogenes constitutive | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? | |
ATP + 2-deoxy-5-keto-D-gluconic acid | involved in myo-inositol degradation | Klebsiella aerogenes constitutive | ADP + 2-deoxy-5-keto-D-gluconic acid 6-phosphate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8.5 | broad, Tris-chloride buffer | Klebsiella aerogenes |
8 | - |
assay at | Klebsiella aerogenes |