Activating Compound | Comment | Organism | Structure |
---|---|---|---|
thrombin | stimulation of the lung epithelial cell line A-549 by thrombin leads to transient increase of SPHK1 activity and elevation of intracellular sphingosine 1-phosphate, abrogation of this stimulation by SPHK1-specific siRNA, pharmacological inhibition, or expression of a dominant-negative SPHK1 mutant blocks the response to thrombin. PAR-1 or thrombin-induced cytokine production and adhesion factor expression of human umbilical vein endothelial cells is also dependent on SPHK1 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
A-549 cell | - |
Homo sapiens | - |
endothelial cell | dermal microvascular endothelial cell | Mus musculus | - |
HUVEC cell | - |
Homo sapiens | - |
General Information | Comment | Organism |
---|---|---|
physiological function | in SPHK1-deficient mice, absence of the enzyme abrogates MCP-1 production induced in dermal microvascular endothelial cells upon treatment with thrombin or PAR-1 activating peptide | Mus musculus |
physiological function | stimulation of the lung epithelial cell line A-549 by thrombin leads to transient increase of SPHK1 activity and elevation of intracellular sphingosine 1-phosphate, abrogation of this stimulation by SPHK1-specific siRNA, pharmacological inhibition, or expression of a dominant-negative SPHK1 mutant blocks the response to thrombin. PAR-1 or thrombin-induced cytokine production and adhesion factor expression of human umbilical vein endothelial cells is also dependent on SPHK1 | Homo sapiens |