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Literature summary for 2.7.1.90 extracted from

  • Phillips, N.F.B.; Li, Z.; Lindmark, D.G.
    Isolation of a pyrophosphate-dependent phosphofructokinase from Hexamita inflata (1997), Mol. Biochem. Parasitol., 90, 377-380.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate no activation Hexamita inflata

Inhibitors

Inhibitors Comment Organism Structure
AMP no inhibition Hexamita inflata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.011
-
diphosphate
-
Hexamita inflata
0.018 0.023 D-fructose 1,6-bisphosphate
-
Hexamita inflata
0.8
-
D-fructose 6-phosphate
-
Hexamita inflata
1.14
-
phosphate
-
Hexamita inflata

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Hexamita inflata

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
67000
-
1 * 67000, SDS-PAGE Hexamita inflata
70000
-
gel filtration Hexamita inflata

Organism

Organism UniProt Comment Textmining
Hexamita inflata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
over 650fold, to homogeneity Hexamita inflata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Hexamita inflata phosphate + D-fructose 1,6-bisphosphate
-
r

Subunits

Subunits Comment Organism
monomer 1 * 67000, SDS-PAGE Hexamita inflata

Synonyms

Synonyms Comment Organism
PPi-PFK
-
Hexamita inflata