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Literature summary for 2.7.1.90 extracted from
- Pyrophosphate-dependent phosphofructokinase of Entamoeba histolytica: molecular cloning, recombinant expression and inhibition by pyrophosphate analogs (1996), Biochem. J., 316, 57-63.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA sequence determination and analysis, functional expression in Escherichia coli BL21(DE3) as His-tagged protein | Entamoeba histolytica |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 3-[N-(2-phenylthioethyl)-N-methylamino]-1-hydroxypropylidene-1,1-bisphosphonate | i.e. CGP 48048, bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica |  |
| alendronate | bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
| clodronate | bisphosphonate, synthetic diphosphate analogue; inhibition of initial growth after inoculation | Entamoeba histolytica | |
| EDTA | complete inhibition of growth at 0.005 mM | Entamoeba histolytica | |
| etidronate | bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
| pamidronate | bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
| risedronate | bisphosphonate, synthetic diphosphate analogue; inhibition of growth | Entamoeba histolytica | |
| zoledronate | bisphosphonate, synthetic diphosphate analogue; inhibition of initial growth after inoculation | Entamoeba histolytica | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.014 | 0.017 | diphosphate | native enzyme, pH 7.0, 25°C | Entamoeba histolytica | |
| 0.018 | - |
diphosphate | recombinant His-tagged enzyme, pH 7.0, 25°C | Entamoeba histolytica | |
| 0.04 | - |
D-fructose 6-phosphate | native enzyme, pH 7.0, 25°C | Entamoeba histolytica | |
| 0.045 | - |
D-fructose 6-phosphate | recombinant His-tagged enzyme, pH 7.0, 25°C | Entamoeba histolytica | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Entamoeba histolytica | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47580 | - |
x * 47580, DNA sequence determination | Entamoeba histolytica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Entamoeba histolytica | Q27651 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native, partial 146fold, further purification leads to inactivation | Entamoeba histolytica |
| recombinant His-tagged protein from Escherichia coli | Entamoeba histolytica |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| trophozoite | - |
Entamoeba histolytica | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.136 | - |
purified recombinant His-tagged enzyme | Entamoeba histolytica |
| 0.24 | - |
partially purified native enzyme | Entamoeba histolytica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Entamoeba histolytica | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47580, DNA sequence determination | Entamoeba histolytica |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PPi-dependent phosphofructokinase | - |
Entamoeba histolytica |
| PPi-PFK | - |
Entamoeba histolytica |
| pyrophosphate-dependent phosphofructokinase | - |
Entamoeba histolytica |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Entamoeba histolytica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Entamoeba histolytica |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.05 | - |
zoledronate | pH 7.0, 25°C | Entamoeba histolytica | |
| 0.05 | - |
3-[N-(2-phenylthioethyl)-N-methylamino]-1-hydroxypropylidene-1,1-bisphosphonate | pH 7.0, 25°C | Entamoeba histolytica | |
| 0.3 | - |
risedronate | pH 7.0, 25°C | Entamoeba histolytica | |
| 0.4 | - |
alendronate | pH 7.0, 25°C | Entamoeba histolytica | |
| 0.56 | - |
etidronate | pH 7.0, 25°C | Entamoeba histolytica | |
| 85 | - |
clodronate | pH 7.0, 25°C | Entamoeba histolytica | |
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