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Literature summary for 2.7.1.90 extracted from

  • Anderson, R.L.; Sabularse, D.C.
    Inorganic pyrophosphate: D-D-fructose-6-phosphate 1-phosphotransferase from mung bean (1982), Methods Enzymol., 90, 91-97.
No PubMed abstract available
Search for more literature for 2.7.1.90

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate
-
 Vigna radiata
D-Glucose 1,6-bisphosphate
-
 Vigna radiata

Inhibitors

Inhibitors Comment Organism Structure
Increased ionic strength e.g. 30 mM KCl, NH4Cl, 10 mM Na2SO4, (NH4)2SO4 or 5 mM sodium phosphate Vigna radiata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Vigna radiata

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
 Vigna radiata
divalent cations required Vigna radiata
divalent cations Mn2+ or Mg2+ Vigna radiata
Mg2+ requirement Vigna radiata
Mn2+ requirement Vigna radiata
Mn2+ less effective than Mg2+ Vigna radiata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Vigna radiata
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Vigna radiata
-
 mung bean
-

Purification (Commentary)

Purification (Comment) Organism
-
 Vigna radiata

Source Tissue

Source Tissue Comment Organism Textmining
seed germinating Vigna radiata
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
12
-
 purified enzyme Vigna radiata

Storage Stability

Storage Stability Organism
-20°C, in 10 mM Tris-acetate buffer, pH 7.3, 0.1 mM EDTA, 0.5 mM DTT, 17 mM KCl, plus 20% v/v glycerol, 6 months Vigna radiata
-80°C, in 10 mM Tris-acetate buffer, pH 7.3, 0.1 mM EDTA, 0.5 mM DTT, 17 mM KCl, 6 months Vigna radiata
4°C, without glycerol, 1 week, then slow decrease of activity over a period of weeks Vigna radiata

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
 Vigna radiata phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Vigna radiata phosphate + D-fructose 1,6-bisphosphate
-
 r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Vigna radiata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 HEPES-NaOH buffer Vigna radiata