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Literature summary for 2.7.1.90 extracted from

  • Kobayashi, M.; Funane, K.; Ohya, S.; Torikoshi, H.; Kurogi, M.; Ishiuchi, D.
    Pyrophosphate-dependent phosphofructokinase: its oligomeric froms in mature green tomato fruit (1992), Biosci. Biotechnol. Biochem., 56, 54-57.
No PubMed abstract available
Search for more literature for 2.7.1.90

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate 11fold Solanum lycopersicum
D-fructose 2,6-bisphosphate forward reaction only Solanum lycopersicum
D-glucose forward reaction Solanum lycopersicum
D-glucose 1-phosphate reverse reaction Solanum lycopersicum

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction Solanum lycopersicum
Cl-
-
 Solanum lycopersicum
Co2+ complete inhibition in the forward reaction, strong inhibition in the reverse reaction Solanum lycopersicum
diphosphate
-
 Solanum lycopersicum
EDTA
-
 Solanum lycopersicum
KCl complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction Solanum lycopersicum
Mn2+ inhibitory in the forward reaction only, slightly activating in the reverse reaction Solanum lycopersicum
NaCl complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction Solanum lycopersicum
NH4+ complete inhibition in the forward reaction, slightly inhibitory in the reverse reaction Solanum lycopersicum
SO42- gluconeogentic reaction Solanum lycopersicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.3 0.38 D-fructose 6-phosphate
-
 Solanum lycopersicum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ no activation of forward reaction Solanum lycopersicum
Co2+ no activitaion Solanum lycopersicum
K+ slightly activating in forward reaction Solanum lycopersicum
Mg2+ requirement Solanum lycopersicum
Mg2+ less effective than Mn2+ Solanum lycopersicum
Mn2+ inhibitory in the forward reaction only, slightly activating in the reverse reaction Solanum lycopersicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
61000 62000 PAGE, gel filtration Solanum lycopersicum
64000
-
 x * 64000 + x * 68000, SDS-PAGE Solanum lycopersicum
68000
-
 x * 64000 + x * 68000, SDS-PAGE Solanum lycopersicum
123000 136000 dimer, PAGE, gel filtration Solanum lycopersicum
243000 262000 predominant tetrameric form, PAGE, gel filtration, in the presence of diphosphate the oligomer dissociates into monomers, D-fructose 2,6-bisphosphate does not reassociate Solanum lycopersicum
425000
-
 hexamer, PAGE, in the presence of diphosphate the oligomer dissociates into monomers, D-fructose 2,6-bisphosphate does not reassociate Solanum lycopersicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Solanum lycopersicum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Solanum lycopersicum
-
 cv. Super First
-

Purification (Commentary)

Purification (Comment) Organism
partial, 569fold Solanum lycopersicum

Source Tissue

Source Tissue Comment Organism Textmining
fruit mature green Solanum lycopersicum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.72
-
 partially purified enzyme Solanum lycopersicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
 Solanum lycopersicum phosphate + D-fructose 1,6-bisphosphate
-
 ?
diphosphate + D-fructose 6-phosphate
-
 Solanum lycopersicum phosphate + D-fructose 1,6-bisphosphate
-
 r

Subunits

Subunits Comment Organism
oligomer x * 64000 + x * 68000, SDS-PAGE Solanum lycopersicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Solanum lycopersicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 assay at Solanum lycopersicum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.3
-
 diphosphate pH 8.0, 25°C Solanum lycopersicum