Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | forward reaction only | Daucus carota |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters, in the absence or presence of D-fructose 2,6-bisphosphate | Daucus carota | |
0.014 | 0.017 | diphosphate | pH 8.0, 25°C | Daucus carota | |
0.13 | - |
D-fructose 6-phosphate | pH 8.0, 25°C | Daucus carota | |
0.5 | - |
Mg2+ | forward reaction, pH 8.0, 25°C | Daucus carota | |
1 | - |
D-fructose 1,6-bisphosphate | reverse reaction, pH 8.0, 25°C | Daucus carota | |
1.7 | - |
phosphate | reverse reaction, pH 8.0, 25°C | Daucus carota | |
1.9 | - |
Mg2+ | reverse reaction, pH 8.0, 25°C | Daucus carota |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Daucus carota |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
2 * 60000 + 2 * 65000-67000, alpha2-beta2, SDS-PAGE | Daucus carota |
294000 | - |
gel filtration, enzyme does not dissociate in the presence of D-fructose 2,6-bisphosphate | Daucus carota |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | Daucus carota | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Daucus carota | - |
- |
- |
Purification (Comment) | Organism |
---|---|
167fold | Daucus carota |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Daucus carota | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2 | - |
purified enzyme | Daucus carota |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | - |
Daucus carota | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + D-fructose 6-phosphate | ratio forward/reverse reaction: 7:1 without D-fructose 2,6-bisphosphate and 1:1 in the presence of this effector | Daucus carota | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Daucus carota | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 60000 + 2 * 65000-67000, alpha2-beta2, SDS-PAGE | Daucus carota |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Daucus carota |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Daucus carota |