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Literature summary for 2.7.1.90 extracted from

  • Macdonald, F.D.; Preiss, J.
    The subcellular location and characteristics of pyrophosphate-D-fructose-6-phosphate 1-phosphotransferase from suspension cultured cells of soybean (1986), Planta, 167, 240-245.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
D-fructose 2,6-bisphosphate dependent on substrate concentrations Glycine max

Inhibitors

Inhibitors Comment Organism Structure
diphosphate
-
Glycine max
phosphate
-
Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetic parameters, in the absence or presence of D-fructose 2,6-bisphosphate Glycine max
3.8 10.6 D-fructose 6-phosphate pH 7.5, 25°C Glycine max
3.8 10.6 D-fructose 6-phosphate with diphosphate Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Glycine max 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ requirement Glycine max

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
128000
-
sucrose density gradient centrifugation Glycine max
183000
-
gel filtration Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Glycine max
-
phosphate + D-fructose 1,6-bisphosphate
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
cv. Mandarin
-

Purification (Commentary)

Purification (Comment) Organism
partial, 102fold Glycine max

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
8.47
-
partially purified enzyme Glycine max

Storage Stability

Storage Stability Organism
-90°C, quick frozen in a dry-ice and methanol bath, at least 6 months Glycine max
0°C, in HEPES-glycol buffer, several days Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
Glycine max phosphate + D-fructose 1,6-bisphosphate
-
?
diphosphate + D-fructose 6-phosphate
-
Glycine max phosphate + D-fructose 1,6-bisphosphate
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Glycine max