Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose 2,6-bisphosphate | dependent on substrate concentrations | Glycine max |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Glycine max | |
phosphate | - |
Glycine max |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetic parameters, in the absence or presence of D-fructose 2,6-bisphosphate | Glycine max | |
3.8 | 10.6 | D-fructose 6-phosphate | pH 7.5, 25°C | Glycine max | |
3.8 | 10.6 | D-fructose 6-phosphate | with diphosphate | Glycine max |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Glycine max | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement | Glycine max |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
128000 | - |
sucrose density gradient centrifugation | Glycine max |
183000 | - |
gel filtration | Glycine max |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | Glycine max | - |
phosphate + D-fructose 1,6-bisphosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Glycine max | - |
cv. Mandarin | - |
Purification (Comment) | Organism |
---|---|
partial, 102fold | Glycine max |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Glycine max | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.47 | - |
partially purified enzyme | Glycine max |
Storage Stability | Organism |
---|---|
-90°C, quick frozen in a dry-ice and methanol bath, at least 6 months | Glycine max |
0°C, in HEPES-glycol buffer, several days | Glycine max |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
diphosphate + D-fructose 6-phosphate | - |
Glycine max | phosphate + D-fructose 1,6-bisphosphate | - |
? | |
diphosphate + D-fructose 6-phosphate | - |
Glycine max | phosphate + D-fructose 1,6-bisphosphate | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Glycine max |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Glycine max |