Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.1.90 extracted from

  • Pfleiderer, C.; Klemme, J.H.
    Pyrophosphate-dependent D-D-fructose-6-phosphate-phosphotransferase in Rhodospirillaceae (1980), Z. Naturforsch. C, 35c, 229-238.
No PubMed abstract available
Search for more literature for 2.7.1.90

Activating Compound

Activating Compound Comment Organism Structure
ATP slight activation of forward reaction Rhodospirillum rubrum
cAMP forward reaction Rhodospirillum rubrum
citrate slight activation of forward reaction Rhodospirillum rubrum

General Stability

General Stability Organism
PAGE or dialysis against Mg-free Tris-HCl buffer, pH 7.5, inactivates Rhodospirillum rubrum

Inhibitors

Inhibitors Comment Organism Structure
ADP kinetics Rhodospirillum rubrum
AMP kinetics Rhodospirillum rubrum
AMP kinetics Rubrivivax gelatinosus
ATP only at low concentrations of MgCl2, reverse reaction Rhodospirillum rubrum
citrate
-
 Rhodospirillum rubrum
D-glucose 6-phosphate weak Rhodospirillum rubrum
D-glucose 6-phosphate weak Rubrivivax gelatinosus
diphosphate reverse reaction Rhodospirillum rubrum
additional information no inhibition by cAMP Rhodospirillum rubrum
phosphoenolpyruvate weak, reverse reaction Rhodospirillum rubrum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018 0.023 D-fructose 1,6-bisphosphate pH 8.6, 30°C Rhodospirillum rubrum
0.025 0.033 diphosphate pH 7.2, 30°C Rhodospirillum rubrum
0.3 0.38 D-fructose 6-phosphate pH 7.2, 30°C Rhodospirillum rubrum
0.59 1 phosphate pH 8.6, 30°C Rhodospirillum rubrum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ no activitaion Rhodospirillum rubrum
Mg2+ requirement Rubrivivax gelatinosus
Mg2+ requirement Rhodospirillum rubrum
Mn2+ not required Rhodospirillum rubrum
additional information no activation by Zn2+ Rhodospirillum rubrum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95000
-
 gel filtration Rhodospirillum rubrum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
diphosphate + D-fructose 6-phosphate Rubrivivax gelatinosus
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate Rhodospirillum rubrum
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate Rhodospirillum rubrum S1
-
 phosphate + D-fructose 1,6-bisphosphate
-
 r

Organism

Organism UniProt Comment Textmining
no activity in Rhodopseudomonas capsulata
-
-
-
no activity in Rhodopseudomonas capsulata Kb1
-
-
-
no activity in Rhodopseudomonas palustris
-
-
-
no activity in Rhodopseudomonas palustris 1a1
-
-
-
no activity in Rhodopseudomonas sphaeroides
-
-
-
no activity in Rhodopseudomonas viridis
-
-
-
Rhodospirillum rubrum
-
-
-
Rhodospirillum rubrum S1
-
-
-
Rubrivivax gelatinosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial 83.4fold Rhodospirillum rubrum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
24.2
-
 partially purified enzyme Rhodospirillum rubrum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
diphosphate + D-fructose 6-phosphate
-
 Rubrivivax gelatinosus phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate
-
 Rhodospirillum rubrum phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Rubrivivax gelatinosus phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Rhodospirillum rubrum phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Rubrivivax gelatinosus phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Rhodospirillum rubrum phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate
-
 Rhodospirillum rubrum S1 phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate Rhodospirillum rubrum S1 phosphate + D-fructose 1,6-bisphosphate
-
 r
diphosphate + D-fructose 6-phosphate specific for diphosphate as phosphor donor Rhodospirillum rubrum S1 phosphate + D-fructose 1,6-bisphosphate
-
 r
phosphate + D-glucose 1,6-bisphosphate reaction at about 70% of the rate with D-fructose 1,6-bisphosphate Rhodospirillum rubrum ?
-
 ?
phosphate + D-glucose 1,6-bisphosphate reaction at about 70% of the rate with D-fructose 1,6-bisphosphate Rhodospirillum rubrum S1 ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Rhodospirillum rubrum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62
-
 10 min stable in the presence of 5 mM MgCl2 Rhodospirillum rubrum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
 forward reaction Rhodospirillum rubrum
8.6
-
 reverse reaction Rhodospirillum rubrum