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Literature summary for 2.7.1.90 extracted from
- Isolation and characterization of a pyrophosphate-dependent phosphofructokinase from Propionibacterium shermanii (1975), J. Biol. Chem., 250, 8690-8695.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | not affected by ATP, AMP, citrate, pyruvate, and malate | Propionibacterium freudenreichii subsp. shermanii |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| AMP | no inhibition | Propionibacterium freudenreichii subsp. shermanii |  |
| ATP | no inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| citrate | no inhibition | Propionibacterium freudenreichii subsp. shermanii | |
| additional information | not affected by ATP, AMP, citrate, pyruvate, and malate | Propionibacterium freudenreichii subsp. shermanii | |
| phosphoenolpyruvate | no inhibition | Propionibacterium freudenreichii subsp. shermanii | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.051 | - |
D-fructose 1,6-bisphosphate | pH 7.4, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 0.064 | - |
Mn2+ | pH 7.4, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 0.069 | - |
diphosphate | pH 7.4, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
| 0.083 | - |
Mg2+ | pH 7.4, 25°C | Propionibacterium freudenreichii subsp. shermanii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Propionibacterium freudenreichii subsp. shermanii | |
| Mn2+ | requirement | Propionibacterium freudenreichii subsp. shermanii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
2 * 48000, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |
| 95000 | - |
high speed meniscus depletion ultracentrifugation | Propionibacterium freudenreichii subsp. shermanii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| diphosphate + D-fructose 6-phosphate | Propionibacterium freudenreichii subsp. shermanii | - |
phosphate + D-fructose 1,6-bisphosphate | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 631fold | Propionibacterium freudenreichii subsp. shermanii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 245 | - |
purified enzyme | Propionibacterium freudenreichii subsp. shermanii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arsenate + D-fructose 1,6-bisphosphate | no substrates are SO42-, MoO42-, SeO42- or ribulose 1,6-bisphosphate | Propionibacterium freudenreichii subsp. shermanii | ? | - |
r | |
| diphosphate + D-fructose 6-phosphate | - |
Propionibacterium freudenreichii subsp. shermanii | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for D-fructose 6-phosphate and D-fructose 1,6-bisphosphate | Propionibacterium freudenreichii subsp. shermanii | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| diphosphate + D-fructose 6-phosphate | specific for diphosphate as phosphor donor | Propionibacterium freudenreichii subsp. shermanii | phosphate + D-fructose 1,6-bisphosphate | - |
r | |
| phosphate + D-glucose 1,6-bisphosphate | no activity | Propionibacterium freudenreichii subsp. shermanii | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 48000, SDS-PAGE | Propionibacterium freudenreichii subsp. shermanii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Propionibacterium freudenreichii subsp. shermanii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
imidazole-HCl buffer, forward and reverse reaction | Propionibacterium freudenreichii subsp. shermanii |
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