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Literature summary for 2.7.1.89 extracted from

  • Luense, C.E.; Scott, F.J.; Suckling, C.J.; Mayer, G.
    Novel TPP-riboswitch activators bypass metabolic enzyme dependency (2014), Front. Chem., 2, 53.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.89

Protein Variants

Protein Variants Comment Organism
additional information construction of a thiK deletion mutant strain, phenotype Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + thiamine Escherichia coli
-
 ADP + thiamine phosphate
-
 ?
additional information Escherichia coli triazolethiamine is likely to be recognized and phosphorylated by endogenous bacterial enzymes, such as ThiK ?
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P75948 gene thiK
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + thiamine
-
 Escherichia coli ADP + thiamine phosphate
-
 ?
additional information triazolethiamine is likely to be recognized and phosphorylated by endogenous bacterial enzymes, such as ThiK Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
ThiK
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli

General Information

General Information Comment Organism
malfunction a thiK deletion strain (DELTAthiK) reveals decreased thiamine sensitivity, since this enzyme is required for the phosphorylation of thiamine to thiamine diphosphate. Deleting the thiamine kinase ThiK abolishes thiamine and triazolethiamine-dependent inhibition of reporter gene expression. The reporter gene expression in the thiK deletion strain is dramatically decreased in the presence of pyrithiamine Escherichia coli
metabolism thiamine is synthesized from two precursors, hydroxymethylpyrimidine diphosphate and hydroxyethylthiazole phosphate, which are produced independently and finally joined to form thiamine phosphate Escherichia coli
physiological function riboswitches are conserved regions within mRNA molecules that bind specific metabolites and regulate gene expression. TPP-riboswitches, which respond to thiamine diophosphate, are involved in the regulation of thiamine metabolism. Thiamine analogues containing a central 1,2,3-triazole group induce repression of thiM-riboswitch dependent gene expression in different Escherichiac coli strains. Triazolethiamine shows concentration-dependent reporter gene repression that is dependent on the presence of thiamine kinase ThiK, whereas the effect of pyrithiamine, a known TPP-riboswitch modulator, is ThiK-independent, overview. ThiK dependency is bypassed by triazolethiamine-derivatives that bear phosphate mimicking and metal-ion chelating moieties Escherichia coli