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Literature summary for 2.7.1.86 extracted from
- Molecular conversion of NAD Kinase to NADH kinase through single amino acid residue substitution (2005), J. Biol. Chem., 280, 24104-24112.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3)pLysS cells | Mycobacterium tuberculosis |
| expressed in Escherichia coli BL21(DE3)pLysS cells | Micrococcus flavus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| G183R | mutant with partially retained NADH kinase activity | Micrococcus flavus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
ATP | wild type enzyme | Micrococcus flavus |  |
| 0.35 | - |
NADH | with tetrapolyphosphate as the phosphate donor, wild type enzyme | Micrococcus flavus | |
| 0.45 | - |
NADH | with ATP as the phosphate donor, wild type enzyme | Micrococcus flavus | |
| 0.47 | - |
ATP | mutant enzyme G183R | Micrococcus flavus | |
| 0.58 | - |
Tetrapolyphosphate | wild type enzyme | Micrococcus flavus | |
| 0.68 | - |
Tetrapolyphosphate | mutant enzyme G183R | Micrococcus flavus | |
| 0.72 | - |
NADH | with tetrapolyphosphate as the phosphate donor, mutant enzyme G183R | Micrococcus flavus | |
| 1.66 | - |
NADH | with ATP as the phosphate donor, mutant enzyme G183R | Micrococcus flavus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Micrococcus flavus | - |
- |
- |
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Butyl-Toyopearl 650M column chromatography | Mycobacterium tuberculosis |
| Butyl-Toyopearl 650M column chromatography | Micrococcus flavus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.22 | - |
in the presence of 5 mM tetrapolyphosphate | Mycobacterium tuberculosis |
| 0.65 | - |
in the presence of 5 mM ATP | Mycobacterium tuberculosis |
| 3.04 | - |
in the presence of 5 mM tetrapolyphosphate | Micrococcus flavus |
| 8.66 | - |
in the presence of 5 mM ATP | Micrococcus flavus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NADH | - |
Mycobacterium tuberculosis | ADP + NADPH | - |
? | |
| ATP + NADH | - |
Micrococcus flavus | ADP + NADPH | - |
? | |
| ATP + NADH | - |
Mycobacterium tuberculosis H37Rv | ADP + NADPH | - |
? | |
| tetrapolyphosphate + NADH | - |
Mycobacterium tuberculosis | tripolyphosphate + NADPH | - |
? | |
| tetrapolyphosphate + NADH | - |
Micrococcus flavus | tripolyphosphate + NADPH | - |
? | |
| tetrapolyphosphate + NADH | - |
Mycobacterium tuberculosis H37Rv | tripolyphosphate + NADPH | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Micrococcus flavus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ATP-NADH kinase | - |
Mycobacterium tuberculosis |
| ATP-NADH kinase | - |
Micrococcus flavus |
| Mfnk | exhibits polyphosphate- and ATP-dependent NADH kinase activities | Micrococcus flavus |
| Ppnk | exhibits polyphosphate- and ATP-dependent NADH kinase activities | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mycobacterium tuberculosis | |
| ATP | - |
Micrococcus flavus | |
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Release 2023.1 (January 2023)
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