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Literature summary for 2.7.1.86 extracted from

  • Ohno, T.; Suzuki, T.; Horiuchi, T.
    Specific amplification of NADH using NADH kinase in a reaction mixture containing excess NAD+ (1994), Biosci. Biotechnol. Biochem., 58, 976-977.
No PubMed abstract available
Search for more literature for 2.7.1.86

Application

Application Comment Organism
analysis the enzyme is useful for amplification of NADH in presence of excess NAD+ and is applicable to sensitive measurement of NAD+ dependent dehydrogenase or ist substrate Pichia membranifaciens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Pichia membranifaciens 5739
-

Organism

Organism UniProt Comment Textmining
Pichia membranifaciens
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 amplification of NADH without the influence of coexisting NAD+, by phosphorylating NADH into NADPH and putting this NAPH through an NADP+-NADPH cycling system Pichia membranifaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + NADH specific for NADH Pichia membranifaciens ADP + NADPH
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 10 min, stable Pichia membranifaciens
35
-
 10 min, 17% loss of activity Pichia membranifaciens
40
-
 10 min, 40% loss of activity Pichia membranifaciens