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Literature summary for 2.7.1.86 extracted from
- Purification and properties of reduced diphosphopyridine nucleotide kinase from yeast mitochondria (1972), J. Biol. Chem., 247, 1473-1478.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetate | high concentrations of carboxylic acid, e.g. acetate required for maximal activity | Saccharomyces cerevisiae |  |
General Stability
| General Stability | Organism |
|---|---|
| ammonium sulfate, 0.2 M protects against denaturation | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ATP | above 5 mM | Saccharomyces cerevisiae | |
| Mg2+ | at 10 mM or above | Saccharomyces cerevisiae | |
| Mn2+ | at 1 mM or above | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.042 | - |
NADH | pH 7.8, 30°C, in presence of 0.2 M sodium acetate | Saccharomyces cerevisiae | |
| 1 | - |
ATP | pH 7.8, 30°C, in presence of 0.2 M sodium acetate | Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Saccharomyces cerevisiae | 5829 | - |
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | either Mn2+ or Mg2+ activate at low concentrations. Km: Km: 1.0 mM | Saccharomyces cerevisiae | |
| Mn2+ | either Mn2+ or Mg2+ activate at low concentrations | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NADH | Saccharomyces cerevisiae | may be involved in maintaining the level of triphosphopyridine nucleotide in the mitochondria | ADP + NADPH | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.279 | - |
- |
Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C or -20°C, 95% loss of activity after overnight storage | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + NADH | specific for NADH | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
| ATP + NADH | may be involved in maintaining the level of triphosphopyridine nucleotide in the mitochondria | Saccharomyces cerevisiae | ADP + NADPH | - |
? | |
| CTP + NADH | 67% of activity with ATP | Saccharomyces cerevisiae | CDP + NADPH | - |
? | |
| GTP + NADH | 45% of activity with ATP | Saccharomyces cerevisiae | GDP + NADPH | - |
? | |
| ITP + NADH | 61% of activity with ATP | Saccharomyces cerevisiae | IDP + NADPH | - |
? | |
| UTP + NADH | 50% of activity with ATP | Saccharomyces cerevisiae | UDP + NADPH | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.9 | 8.6 | - |
Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.2 | 9.9 | pH 6.2: about 35% of maximal activity, pH 9.9: about 50% of maximal activity | Saccharomyces cerevisiae |
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