KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.6 | - |
arbutin | pH 7.5 | Klebsiella aerogenes | |
1 | - |
cellobiose | pH 7.5 | Klebsiella aerogenes | |
1.5 | - |
gentiobiose | pH 7.5 | Klebsiella aerogenes | |
1.7 | - |
ATP | pH 7.5 | Klebsiella aerogenes | |
2.5 | - |
Salicin | pH 7.5 | Klebsiella aerogenes | |
2.5 | - |
phenyl beta-D-glucoside | pH 7.5 | Klebsiella aerogenes | |
3.1 | - |
amygdalin | pH 7.5 | Klebsiella aerogenes | |
3.1 | - |
sophorose | pH 7.5 | Klebsiella aerogenes | |
4 | - |
cellobiitol | pH 7.5 | Klebsiella aerogenes | |
6.3 | - |
cellotetraose | pH 7.5 | Klebsiella aerogenes | |
6.7 | - |
cellotriose | pH 7.5 | Klebsiella aerogenes | |
13.1 | - |
methyl beta-D-glucoside | pH 7.5 | Klebsiella aerogenes |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
150000 | - |
sucrose density gradient centrifugation | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + cellobiose | Klebsiella aerogenes | first reaction in metabolism of cellobiose, inducible enzyme | ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella aerogenes | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Klebsiella aerogenes |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Klebsiella aerogenes |
Storage Stability | Organism |
---|---|
4°C, 35% loss of activity after 2 months | Klebsiella aerogenes |
frozen, 50% loss of activity after 2 weeks | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + amygdalin | 21% of the activity with cellobiose | Klebsiella aerogenes | ADP + amygdalin 6-phosphate | - |
? | |
ATP + arbutin | 36% of the activity with cellobiose | Klebsiella aerogenes | ADP + arbutin 6-phosphate | - |
? | |
ATP + cellobiitol | 25% of the activity with cellobiose | Klebsiella aerogenes | ? | - |
? | |
ATP + cellobiose | - |
Klebsiella aerogenes | ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose | - |
? | |
ATP + cellobiose | first reaction in metabolism of cellobiose, inducible enzyme | Klebsiella aerogenes | ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose | - |
? | |
ATP + cellotetraose | 33% of the activity with cellobiose | Klebsiella aerogenes | ADP + 6-phospho-beta-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-D-glucose | - |
? | |
ATP + cellotriose | 47% of the activity with cellobiose | Klebsiella aerogenes | ADP + 6-phospho-beta-D-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-D-glucose | - |
? | |
ATP + gentiobiose | 70% of the activity with cellobiose | Klebsiella aerogenes | ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose | - |
? | |
ATP + methyl beta-D-glucoside | as active as cellobiose | Klebsiella aerogenes | ADP + methyl beta-D-glucoside 6-phosphate | - |
? | |
ATP + phenyl beta-D-glucoside | 70% of the activity with cellobiose | Klebsiella aerogenes | ADP + phenyl beta-D-glucoside 6-phosphate | - |
? | |
ATP + salicin | 87% of the activity with cellobiose | Klebsiella aerogenes | ADP + salicin 6-phosphate | - |
? | |
ATP + sophorose | 19% of the activity with cellobiose | Klebsiella aerogenes | ADP + 6-phospho-beta-D-glucopyranosyl-(1,2)-D-glucose | - |
? | |
CTP + cellobiose | 12% of the activity with ATP | Klebsiella aerogenes | CDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose | - |
? | |
GTP + cellobiose | 15% of the activity with ATP | Klebsiella aerogenes | GDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose | - |
? | |
ITP + cellobiose | 7% of the activity with ATP | Klebsiella aerogenes | IDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose | - |
? | |
additional information | not: ADP, acetyl phosphate, alpha-glycerophosphate, D-fructose, 1,6-diphosphate, phosphoramidate, choline phosphate, creatine phosphate, 3-phosphoglycerate, phenylphosphate, phosphoenolpyruvate | Klebsiella aerogenes | ? | - |
? | |
UTP + cellobiose | 3% of the activity with ATP | Klebsiella aerogenes | UDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
half-life: 10 min | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
MES buffer | Klebsiella aerogenes |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.2 | 8.2 | pH 6.2: about 60% of maximal activity in Mes buffer, pH 8.2: reaction in PIPES buffer, about 70% of maximal activity in MES buffer | Klebsiella aerogenes |