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Literature summary for 2.7.1.85 extracted from

  • Palmer, R.E.; Anderson, R.L.
    Cellobiose metabolism in Aerobacter aerogenes. II. Phosphorylation of cellobiose with adenosine 5-triphosphate by alpha-glucoside kinase (1972), J. Biol. Chem., 247, 3415-3419.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
arbutin pH 7.5 Klebsiella aerogenes
1
-
cellobiose pH 7.5 Klebsiella aerogenes
1.5
-
gentiobiose pH 7.5 Klebsiella aerogenes
1.7
-
ATP pH 7.5 Klebsiella aerogenes
2.5
-
Salicin pH 7.5 Klebsiella aerogenes
2.5
-
phenyl beta-D-glucoside pH 7.5 Klebsiella aerogenes
3.1
-
amygdalin pH 7.5 Klebsiella aerogenes
3.1
-
sophorose pH 7.5 Klebsiella aerogenes
4
-
cellobiitol pH 7.5 Klebsiella aerogenes
6.3
-
cellotetraose pH 7.5 Klebsiella aerogenes
6.7
-
cellotriose pH 7.5 Klebsiella aerogenes
13.1
-
methyl beta-D-glucoside pH 7.5 Klebsiella aerogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
150000
-
sucrose density gradient centrifugation Klebsiella aerogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + cellobiose Klebsiella aerogenes first reaction in metabolism of cellobiose, inducible enzyme ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?

Organism

Organism UniProt Comment Textmining
Klebsiella aerogenes
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Klebsiella aerogenes

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
45
-
-
Klebsiella aerogenes

Storage Stability

Storage Stability Organism
4°C, 35% loss of activity after 2 months Klebsiella aerogenes
frozen, 50% loss of activity after 2 weeks Klebsiella aerogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + amygdalin 21% of the activity with cellobiose Klebsiella aerogenes ADP + amygdalin 6-phosphate
-
?
ATP + arbutin 36% of the activity with cellobiose Klebsiella aerogenes ADP + arbutin 6-phosphate
-
?
ATP + cellobiitol 25% of the activity with cellobiose Klebsiella aerogenes ?
-
?
ATP + cellobiose
-
Klebsiella aerogenes ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
ATP + cellobiose first reaction in metabolism of cellobiose, inducible enzyme Klebsiella aerogenes ADP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
ATP + cellotetraose 33% of the activity with cellobiose Klebsiella aerogenes ADP + 6-phospho-beta-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-D-glucose
-
?
ATP + cellotriose 47% of the activity with cellobiose Klebsiella aerogenes ADP + 6-phospho-beta-D-glucosyl-(1,4)-beta-D-glucosyl-(1,4)-D-glucose
-
?
ATP + gentiobiose 70% of the activity with cellobiose Klebsiella aerogenes ADP + 6-phospho-beta-D-glucosyl-(1,6)-D-glucose
-
?
ATP + methyl beta-D-glucoside as active as cellobiose Klebsiella aerogenes ADP + methyl beta-D-glucoside 6-phosphate
-
?
ATP + phenyl beta-D-glucoside 70% of the activity with cellobiose Klebsiella aerogenes ADP + phenyl beta-D-glucoside 6-phosphate
-
?
ATP + salicin 87% of the activity with cellobiose Klebsiella aerogenes ADP + salicin 6-phosphate
-
?
ATP + sophorose 19% of the activity with cellobiose Klebsiella aerogenes ADP + 6-phospho-beta-D-glucopyranosyl-(1,2)-D-glucose
-
?
CTP + cellobiose 12% of the activity with ATP Klebsiella aerogenes CDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
GTP + cellobiose 15% of the activity with ATP Klebsiella aerogenes GDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
ITP + cellobiose 7% of the activity with ATP Klebsiella aerogenes IDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?
additional information not: ADP, acetyl phosphate, alpha-glycerophosphate, D-fructose, 1,6-diphosphate, phosphoramidate, choline phosphate, creatine phosphate, 3-phosphoglycerate, phenylphosphate, phosphoenolpyruvate Klebsiella aerogenes ?
-
?
UTP + cellobiose 3% of the activity with ATP Klebsiella aerogenes UDP + 6-phospho-beta-D-glucosyl-(1,4)-D-glucose
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
half-life: 10 min Klebsiella aerogenes

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
MES buffer Klebsiella aerogenes

pH Range

pH Minimum pH Maximum Comment Organism
6.2 8.2 pH 6.2: about 60% of maximal activity in Mes buffer, pH 8.2: reaction in PIPES buffer, about 70% of maximal activity in MES buffer Klebsiella aerogenes