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Literature summary for 2.7.1.83 extracted from

  • Preumont, A.; Snoussi, K.; Stroobant, V.; Collet, J.F.; Van Schaftingen, E.
    Molecular identification of pseudouridine-metabolizing enzymes (2008), J. Biol. Chem., 283, 25238-25246.
    View publication on PubMed
Search for more literature for 2.7.1.83

Cloned(Commentary)

Cloned (Comment) Organism
-
 Escherichia coli

Protein Variants

Protein Variants Comment Organism
additional information mutants lacking YeiC activity are unable to metabolize pseudouridine Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
 pseudouridine pH 7.1, 30°C Escherichia coli
0.33
-
 ATP pH 7.1, 30°C Escherichia coli

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Escherichia coli
Mg2+ enzyme is dependent on the presence of Mg2+ Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + pseudouridine Escherichia coli two enzymes are involved in the metabolism of pseudouridine in uropathogenic Escherichia coli, the principal agent of urinary tract infections in humans. The first enzyme, coded by the gene yeiC, specifically phosphorylates pseudouridine to pseudouridine 5'-phosphate. yeiC- mutants are unable to metabolize pseudouridine, in contrast to wild-type Escherichia coli UTI89. The second enzyme, encoded by the gene yeiN (EC 4.2.1.70) belonging to the same operon as yeiC, catalyzes the conversion of pseudouridine 5'-phosphate to uracil and ribose 5-phosphate in a divalent cation-dependent manner ADP + pseudouridine 5'-phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli P30235 strain UTI89
-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + pseudouridine
-
 Escherichia coli ADP + pseudouridine 5'-monophosphate
-
 ?
ATP + pseudouridine
-
 Escherichia coli ADP + pseudouridine 5'-phosphate
-
 ?
ATP + pseudouridine two enzymes are involved in the metabolism of pseudouridine in uropathogenic Escherichia coli, the principal agent of urinary tract infections in humans. The first enzyme, coded by the gene yeiC, specifically phosphorylates pseudouridine to pseudouridine 5'-phosphate. yeiC- mutants are unable to metabolize pseudouridine, in contrast to wild-type Escherichia coli UTI89. The second enzyme, encoded by the gene yeiN (EC 4.2.1.70) belonging to the same operon as yeiC, catalyzes the conversion of pseudouridine 5'-phosphate to uracil and ribose 5-phosphate in a divalent cation-dependent manner Escherichia coli ADP + pseudouridine 5'-phosphate
-
 ?
additional information no activity with: uridine, adenosine, guanosine, or cytidine Escherichia coli ?
-
 ?

Synonyms

Synonyms Comment Organism
YeiC
-
 Escherichia coli