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Literature summary for 2.7.1.78 extracted from

  • Das, U.; Wang, L.K.; Smith, P.; Munir, A.; Shuman, S.
    Structures of bacterial polynucleotide kinase in a Michaelis complex with nucleoside triphosphate (NTP)-Mg2+ and 5-OH RNA and a mixed substrate-product complex with NTP-Mg2+ and a 5-phosphorylated oligonucleotide (2014), J. Bacteriol., 196, 4285-4292.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.1.78

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Acetivibrio thermocellus

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme D38N in complex with GTP-Mg2+ and a 5'-OH RNA oligonucleotide, hanging drop vapor diffusion method, using 100 mM sodium citrate (pH 5.0), 100 mM MgCl2, 18-24% (w/v) PEG-6000 Acetivibrio thermocellus

Protein Variants

Protein Variants Comment Organism
Q51A the mutation reduces kinase specific activity about 3fold Acetivibrio thermocellus
S37A/T80A the double mutation reduces kinase activity 50fold Acetivibrio thermocellus
T54A the mutation reduces kinase specific activity about 3fold Acetivibrio thermocellus
V129A the mutation reduces kinase specific activity about 3fold Acetivibrio thermocellus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Acetivibrio thermocellus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-DNA Acetivibrio thermocellus the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83 ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA Acetivibrio thermocellus DSM 1237 the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83 ADP + 5'-phospho-DNA
-
 ?

Organism

Organism UniProt Comment Textmining
Acetivibrio thermocellus A3DJ38
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-
Acetivibrio thermocellus DSM 1237 A3DJ38
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-

Purification (Commentary)

Purification (Comment) Organism
Ni-agarose column chromatography and Superdex 200 gel filtration Acetivibrio thermocellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-DNA the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83 Acetivibrio thermocellus ADP + 5'-phospho-DNA
-
 ?
ATP + 5'-dephospho-DNA the enzyme makes hydrogen bonds to the ribose 2'-hydroxyls of the 5'-terminal nucleoside, via Gln51, and the penultimate nucleoside, via Gln83 Acetivibrio thermocellus DSM 1237 ADP + 5'-phospho-DNA
-
 ?

Subunits

Subunits Comment Organism
homodimer x-ray crystallography Acetivibrio thermocellus

Synonyms

Synonyms Comment Organism
PNK
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 Acetivibrio thermocellus
polynucleotide kinase
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 Acetivibrio thermocellus